HBV感染型原发性肝癌患者肿瘤微环境中CD4^+、CD8^+T细胞和VEGF水平变化特征及其临床意义  

Changes in CD4^+,CD8^+T Cells and VEGF Levels in Tumor Microenvironment ofPatients with HBV Infected Primary Liver Cancer and Their Clinical Significance

作　　者：方菊梅 张万里 陈怡发 刘黎明[1] 黄庆勇 FANG Jumei;ZHANG Wanli;CHEN Yifa(Ezhou Central Hospital,Ezhou,436000)

机构地区：[1]湖北省鄂州市中心医院,436000 [2]华中科技大学同济医学院附属协和医院,430022

出　　处：《实用癌症杂志》2020年第5期740-744,共5页The Practical Journal of Cancer

摘　　要：目的探究慢性乙肝炎病毒(HBV)感染型研发型肝癌患者肿瘤微环境中CD4^+、CD8^+T细胞、血管内皮生长因子水平变化特点以及临床意义。方法选取94例原发性肝癌患者作为研究对象,按照是否感染HBV分为观察组和对照组,观察组54例患者为HBV感染型原发性肝癌,对照组40例为未感染HBV型原发性肝癌患者。对所有患者的肝穿刺活检标本或肝癌根治切除标本检测,比较分析两组患者肿瘤组织CD4^+T淋巴细胞(CD4^+)、CD8^+T淋巴细胞(CD8^+)的表达情况以及血管内皮因子(VEGF)水平变化情况。结果观察组的CD4^+T细胞水平(55.6%)明显高于对照组的(10.0%),而CD8^+T细胞水平(44.4%)明显低于对照组的(90.0%),差异具有统计学意义(χ^2=20.7,P<0.05);观察组血清VEGF水平[(588.7±148.4)ng/l]显著高于对照组[(217.0±94.3)ng/l],差异具有统计学意义(P<0.001)。结论T细胞的亚群表达情况、血清VEGF与HBV感染型原发性肝癌病情发展和转移有着一定重要意义,能够了解患者的机体免疫情况和病情转移情况,具有良好的临床研究价值。Objective To explore the changes of CD4^+,CD8^+C cells and vascular endothelial growth factor levels and their clinical significance in tumor microenvironment of patients with HBV infected rd-type liver cancer.Methods A total of 94 patients with primary liver cancer were selected as the research objects.According to whether they were infected with HBV,54 patients in the observation group were HBV-infected primary liver cancer,and 40 patients in the control group were uninfected with HBV-type primary liver cancer.The expression of CD4^+T lymphocytes(CD4^+),CD8^+T lymphocytes(CD8^+)and vascular endothelial factor(VEGF)in tumor tissues of the 2 groups were compared and analyzed for the detection of liver biopsy specimens or liver cancer radical resection specimens of all patients.Results The proportion of CD4^+T cells(55.6%)in the observation group infected with HBV was significantly higher than that of the control group(10.0%),while the proportion of CD8^+T cells(44.4%)was lower than that of the control group(90.0%).The serum VEGF level(588.7±148.4)of the observation group was significantly higher than that of the control group(217.0±94.3),and the difference was statistically significant(P<0.001).Conclusion The subgroup expression of T cells,the disease development and metastasis of serum VEGF and HBV infected primary hepatocellular carcinoma are of certain significance.It is of good clinical value to understand the patient's immune status and disease metastasis.

关 键 词：HBV感染 原发性肝癌肿瘤患者 CD4^+T淋巴细胞 CD8^+T淋巴细胞 血管内皮因子 

分 类 号：R735.7[医药卫生—肿瘤]