阳和化岩汤对乳腺癌癌前病变细胞Fas、Caspase-3和Caspase-8表达的影响  

Effect of Yanghe Huayan decoction on the expression of Fas,Caspase-3 and Caspase-8 in breast cancer precancerous cells

作　　者：孙颖 刘晓菲[1,2] 王晓娜 李斐斐 史文娴 郭光鑫 SUN Ying;LIU Xiaofei;WANG Xiaona;LI Feifei;SHI Wenxian;GUO Guangxin(The First Clinical College of Shandong University of Traditional Chinese Medicine, Jinan 250335, Shandong, China;Collaborative Innovation Center of Classical Decoction of Traditional Chinese Medicine of Shandong Province, Jinan 250335, Shandong, China;School of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong, China)

机构地区：[1]山东中医药大学第一临床学院,山东济南250335 [2]山东省中医经典名方协同创新中心,山东济南250335 [3]山东中医药大学中医学院,山东济南250014

出　　处：《现代中西医结合杂志》2020年第17期1825-1829,共5页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(81403408);山东省医药科技发展计划项目(2018WS191);山东省中医经典名方协同创新中心开放课题(2018KFY10)。

摘　　要：目的体外实验观察阳和化岩汤对乳腺癌癌前病变MCF-10AT细胞Fas、Caspase-3和Caspase-8表达的影响,探讨阳和化岩汤对乳腺癌癌前病变的干预作用机制。方法制备阳和化岩汤及其拆方肠吸收液,将MCF-10AT细胞分为7组,阳和化岩汤组、温阳组、活血组、化痰组、解毒组、三苯氧胺组给予相应肠吸收液培养,空白对照组常规培养。培养72 h后,应用Western blot法检测细胞凋亡调控因子Fas、Caspase-3和Caspase-8蛋白表达量,采用RT-PCR法检测Fas、Caspase-3和Caspase-8 mRNA相对表达量。结果阳和化岩汤、三苯氧胺组Fas、Caspase-3和Caspase-8蛋白和mRNA表达量均明显高于空白对照组(P均<0.05),阳和化岩汤、三苯氧胺组比较差异均无统计学意义(P均>0.05)。结论阳和化岩汤能通过死亡受体途径抑制MCF-10AT细胞增殖及诱导凋亡,能够阻断乳腺癌癌变的进展。Objective It is to observe the effect of Yanghe Huayan decoction on the expression of Fas,Caspase-3 and Caspase-8 in MCF-10AT cells of breast cancer precancerous lesions in vitro,and to explore the intervention mechanism of Yanghe Huayan decoction on breast cancer precancerous lesions.Methods Yanghe Huayan Decoction and its split intestinal absorption liquid were prepared,the MCF-10AT cells were divided into 7 groups:Yanghe Huayan decoction group,Wenyang group,Huoxue group,Huatan group,Jiedu group,tamoxifen group which were treated with corresponding intestinal absorption liquid culture,blank control group treated with routine culture.After 72 hours of culture,Western blot was used to detect the expression of apoptosis regulator Fas,Caspase-3 and Caspase-8 protein,and RT-PCR was used to detect the relative expression of Fas,Caspase-3 and Caspase-8 mRNA.Results The protein and mRNA expressions of Fas,Caspase-3 and Caspase-8 in Yanghe Huayan decoction and tamoxifen group were significantly higher than that in the blank control group(all P<0.05).There was no statistical significant difference between Yanghe Huayan decoction and tamoxifen group(all P>0.05).Conclusion Yanghe Huayan decoction can inhibit the proliferation and induce apoptosis of MCF-10AT cells through the death receptor pathway,and can block the progression of breast cancer.

关 键 词：阳和化岩汤 乳腺癌癌前病变 细胞凋亡 FAS CASPASE-3 CASPASE-8 

分 类 号：R737.9[医药卫生—肿瘤]