TREM-1在大鼠脑出血后血肿周围脑组织中的表达研究  

Expression of TREM-1 in brain tissue around hematomas after intracerebral hemorrhage in rats

作　　者：段红玲 孙新刚[2] 张咪咪[1] 侯雅芝 DUAN Hongling;SUN Xingang;ZHANG Mimi;HOU Yazhi(Shanxi Medical University,Taiyuan 030001,China;Second Hospital of Shanxi Medical University,Taiyuan 030001,China;Shanxi Cardiovascular Hospital,Taiyuan 030001,China)

机构地区：[1]山西医科大学,山西太原030001 [2]山西医科大学第二医院,山西太原030001 [3]山西省心血管病医院,山西太原030024

出　　处：《临床医药实践》2020年第6期435-436,480,共3页Proceeding of Clinical Medicine

基　　金：山西省应用基础研究项目(项目编号:201701D221269)。

摘　　要：目的:观察髓系细胞触发受体-1(TREM-1)在大鼠脑出血(ICH)后血肿周围脑组织中的表达及其与脑组织炎症反应形成的关系。方法:选择健康雄性SD大鼠36只,采用随机数字表法分为假手术组和ICH后1 d,2 d,3 d,5 d,7 d组,每组6只。采用自体注血方法制作大鼠ICH后1 d,2 d,3 d,5 d和7 d组模型,以实时荧光定量PCR(RT-PCR)法检测出血后不同时间点血肿周围脑组织中TREM-1的表达及其高峰时间点脑组织中白细胞介素-1β(IL-1β)肿瘤坏死因子-α(TNF-α)的表达。结果:与假手术组相比,各ICH组大鼠脑组织中TREM-1均表达升高,并于ICH后3 d时达到高峰,且与IL-1β及TNF-α的表达呈正相关。结论:TREM-1在大鼠ICH后血肿周围脑组织中呈动态表达升高,并与ICH后脑组织炎症反应密切相关。Objective:To observe the expression of the triggering receptor expressed on myeloid cell-1(TREM-1)in the peripheral brain of the hematoma after spontaneous intracerebral hemorrhage(ICH)and its relationship with the inflammatory response of the brain tissue.Methods:The rat model was established by autologous blood injection on the 1st day,2nd day,3rd day,5th day and 7th day after ICH.The expression of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in the brain tissue at different time points after hemorrhage were detected by real-time quantitative polymerase chain reaction(RT-PCR)method.Results:Compared with the sham operation group,the expression of TREM-1 in the brain tissue of each ICH group increased and reached the peak at 3 days after ICH,and was positively correlated with the expression of IL-1βand TNF-α.Conclusion:The dynamic expression of TREM-1 in the surrounding brain tissue of rats after ICH was increased,which was closely related to the inflammatory reaction of brain tissue after ICH.

关 键 词：自发性脑出血 髓系细胞触发受体-1 炎性反应 

分 类 号：R743.34[医药卫生—神经病学与精神病学]