CTAB反胶束体系合成己酸乙酯及气相色谱法检测  

Ethyl Caproate Synthesis in CTAB Reverse Micelle System and Determination of Gas Chromatography

作　　者：丛懿洁 CONG Yijie(Weihai Ocean Vocational College,Weihai 264300,Shandong,Chin)

机构地区：[1]威海海洋职业学院,山东威海264300

出　　处：《汕头大学学报（自然科学版）》2020年第2期11-20,共10页Journal of Shantou University：Natural Science Edition

基　　金：威海市特色果蔬高值加工工程技术研究中心2019年度科研开放专项资金项目(GSGC-2019-0005)。

摘　　要：试验采用反胶束体系进行己酸乙酯的猪胰脂肪酶催化合成,并用气相色谱检测.己酸乙酯最佳转化条件为:温度为35℃,酶质量浓度为0.003 g/L,己酸浓度为0.3 mol/L,醇酸比为1∶1.气相色谱检测采用外标法定量.结果表明,己酸乙酯色谱纯含量在0.1~1.0μL范围内呈良好线性关系,相关系数为0.9909;己酸乙酯的回收率在87.47%~95.76%;相对标准偏差RSD为2.98%.说明该方法能够准确对反胶束体系脂肪酶催化合成的己酸乙酯进行定性和定量检测.Ethyl caproate was synthesized from caproic acid and anhydrous ethanol catalyzed by porcine pancreas lipase in reversed micellar systems. The optimal reaction conditions: reaction temperature 35 ℃;enzyme concentration 0.003 g/L;ratio of ethanol to acid 1:1;caproic acid concentration 0.3 mol/L. External standard quantitative method was used for gas chromatography.The results showed that the linear scopes were between 0.1 μL and 1.0 μL(R=0.9859);The recovery rate of GC Detection was between 87.47% and 95.76% with the Relative Standard Deviation is 2.98%. The method can be simple, fast and accurate to detect ethyl caproate contents synthesized in reversed micellar systems.

关 键 词：反胶束体系 条件优化 己酸乙酯 气相色谱检测 

分 类 号：TS261[轻工技术与工程—发酵工程]