两株淡水湖泊常见沉水植物共培养对斜生栅藻的抑制作用  

The inhibition effect of two common limnetic submerged macrophytes on Scenedesmus obliquus under co-culture condition

作　　者：马浩天 张飞 张宏江 史飞飞[1] 崔红利 李润植[1] MA Hao-tian;ZHANG Fei;ZHANG Hong-jiang;SHI Fei-fei;CUI Hong-li;LI Run-zhi(Institute of Molecular Agriculture and Bioenergy,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学分子农业与生物能源研究所,太谷030801

出　　处：《生物学杂志》2020年第3期72-75,共4页Journal of Biology

基　　金：国家自然科学基金(30971806,31201266,31401430);国家“948”项目(2014-Z39);山西省煤基重点科技攻关项目(FT-2014-01);山西省重点科技项目(201603D312005)。

摘　　要：沉水植物与微生物藻类是水生生态环境的重要组成部分,二者可能存在竞争关系,而沉水植物往往可以利用化感作用获得优势。为探究沉水植物对淡水微藻的化感抑制作用,选择斜生栅藻作为受试生物,探究了金鱼藻、箬叶藻(马来眼子菜)及其混合培养对斜生微藻生长和生理表型的影响。结果表明,在与沉水植物共培养条件下,斜生栅藻的生长受到显著的抑制作用,在箬叶藻处理下的抑制率最高可达95.7%,并且叶绿素含量下降、丙二醛、抗氧化酶活性升高,其叶绿素含量下降、叶绿素自发荧光改变提示光合系统的破坏,丙二醛、抗氧化酶活性升高表征了抗氧化系统的受损,证明两种沉水植物及其混培均有强烈的抑藻作用,可导致藻细胞出现一系列的受损,并且箬叶藻(马来眼子菜)的作用更强。为确定化感抑制作用机理的探究、相关表型的分析、沉水植物与微藻的生态学关系研究提供了科学基础。Submerged macrophytes and microalgae are important components of the aquatic ecosystem. There may be a competition between them, and submerged plants can often take advantage by allelopathic effects. In order to explore the allelopathic inhibition effect of submerged plants on freshwater microalgae, Scenedesmus obliquus was selected as the test organism, the growth and physiology properties were detected under the Potamogeton Malaianus, Ceratophyllum demersum and their mixed culture condition. The results showed that under the condition of co-culture with submerged plants, the growth of S. obliquus was significantly inhibited, and the chlorophyll content decreased, the chlorophyll autofluorescence changed, which probed the damage of microalgal photosynthetic system, and the increase of malondialdehyde and antioxidant enzymes activities could illustrate the damage of the antioxidant system. All the results proved that both submerged plants and their mixed cultures had strong algae-inhibiting effects, which could lead to a series of damage to algae cells. The above research results would provide a scientific basis for the investigation of the mechanism of allelopathic inhibition, the analysis of related phenotypes, and the ecological relationship between submerged plants and microalgae.

关 键 词：沉水植物 斜生栅藻 生长抑制 生理表型 

分 类 号：X171.5[环境科学与工程—环境科学]