基于钴60的大鼠BMSCs细胞实验模型的建立  

The establishment of rat BMSCs radiation model based on Co 60

作　　者：赵路 贾骏麒 田磊 靳丹 宗春琳 莫宏兵[1] ZHAO Lu;JIA Junqi;TIAN Lei;JIN Dan;ZONG Chunlin;MO Hongbing(Hospital of Stomatology,Jiamusi University,154007 China;State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Clinical Research Center for Oral Diseases,Department of Oral and Maxillofacial Surgery,School of Stomatology,the Fourth Military Medical University,China)

机构地区：[1]佳木斯大学口腔医院,154007 [2]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔疾病临床医学研究中心,第四军医大学口腔医院口腔颌面外科

出　　处：《实用口腔医学杂志》2020年第3期433-436,共4页Journal of Practical Stomatology

基　　金：国家自然科学基金面上项目(编号:81670964)。

摘　　要：目的:探索基于钴60放射源的γ射线用于建立大鼠骨髓间充质干细胞实验模型的理想加载条件。方法:分别应用γ射线和X射线对大鼠骨髓间充质干细胞(BMSCs)进行梯度剂量(2~8 Gy)照射,照射后检测各剂量下细胞增殖能力和凋亡程度;并检测照射后各剂量下活细胞比例和NOX4合成量,进行组间比较。结果:4 Gy以上剂量γ射线可造成细胞形态、增殖能力下降、凋亡增加;4 Gy剂量亚组活细胞明显高于6、8 Gy剂量亚组;相同剂量的γ和X射线在抑制细胞增殖、促细胞凋亡、促NOX4合成等方面无显著性差异。结论:利用钴60放射源的γ射线在4 Gy剂量、2.538 Gy/min剂量率照射条件下可建立大鼠骨髓间充质干细胞实验模型,与同等剂量的X射线具有较好的等效关系。Objective:To explore the ideal loading conditions for the establishment of experimental model of rat bone marrow mesenchymal stem cells(BMSCs)based onγ-ray of Co 60.Methods:BMSCs of rats were irradiated byγ-ray and X-ray at 2-8 Gy respectively.After irradiation,the proliferation and apoptosis of the cells were detected by CCK-8 assay and TUNEL staining respectively,the ratio of viable cells and the amount of NOX4 synthesis after irradiation were measured by immunofluorescence technique and LCSM respectively.Results:When the dose ofγ-ray was more than 4 Gy,the cell morphology,proliferation and apoptosis were significantly inhibited;the living cell stock of 4 Gy group was significantly higher than that of 6 Gy and 8 Gy group;there was no significant difference betweenγ-ray and X-ray at the same dosage in inhibiting cell proliferation,promoting cell apoptosis and promoting NOX4 synthesis.Conclusion:The experimental model of BMSCs can be established under the irradiation conditions of 4 Gy dose and 2.538 Gy/min dose rate usingγ-ray of Co 60 radiation source,which has a good equivalent relationship with the same dose of X-ray.

关 键 词：骨髓间充质干细胞(BMSCs) Γ射线 X射线 钴60 

分 类 号：R739.8[医药卫生—肿瘤] R815.5[医药卫生—临床医学]