不同盐度下脊尾白虾proPO和SOD基因表达及其酶活力分析  

Enzyme activity and gene expressions of immune-related in Exopalaemon carinicauda following long-term different salinity exposure

作　　者：许振山 张静 张伟丽 梁俊平 李健[2] 李吉涛[2] Xu Zhenshan;Zhang Jing;Zhang Weili;Liang Junpingu;Li Jian;Li Jitao(College of Fisheries,Henan Normal University,Xinxiang Henan 453007;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture,Qiaodao Shandong 266071)

机构地区：[1]河南师范大学水产学院,河南新乡453007 [2]中国水产科学研究院黄海水产研究所,农业部海洋渔业可持续发展重点实验室,山东青岛266071

出　　处：《河南水产》2020年第1期17-20,共4页HENAN FISHERIES

基　　金：国家虾产业技术体系(CARS-48);河南省科技攻关项目(202102110257)。

摘　　要：脊尾白虾对盐度具有较强的适应性,为了了解长期生活于不同盐度下脊尾白虾的免疫防御能力,选择生活在盐度5、 10、 15、 20、 25、 30水体中的脊尾白虾幼虾,利用荧光定量PCR检测了脊尾白虾肝胰腺酚氧化酶原(proPO)基因和超氧化物歧化酶(SOD)基因表达水平,同时测定了肝胰腺酚氧化酶(PO)和SOD活力。结果显示,随着盐度升高,脊尾白虾PO和SOD活力呈现先升高后降低趋势,在盐度15、 20时的酶活力显著高于其它盐度组,proPO基因和SOD基因表达量变化趋势也呈现与酶活力变化相同趋势。结果说明,生长在不同盐度水体中的脊尾白虾,免疫力差异显著,在盐度15、 20水体中具有较强免疫力。Exopalaemon carinicauda has a good environmental adaptability,it can be reared seawater so in order to investigate immune-related enzyme activity of E.carinicauda at different salinity,phenofoxidase,superoxide dismutase activity and gene expressions of prophenoloxidase and superoxide dismutase were examined in E.carinicauda juveniles which had been reared at different salinity levels of 5,10,15,20,25 and 30.The results showed that the enzyme activity of phenofoxidase and superoxide dismutase initially increased,but then decreased with increasing salinity,the enzyme activity were significantly higher at salinity 15 and 20 than other salinity groups,and the gene expressions of prophenoloxidase and superoxide dismutase were significantly higher at salinity 15 and 20 than other salinity groups.It was concluded that following long-term exposure to 15 and 20 seawater,E.carinicauda juveniles exhibited increased resistance.

关 键 词：脊尾白虾 酶活力 基因表达 盐度 

分 类 号：S917.4[农业科学—水产科学]