人头颈鳞癌中ceRNA网络构建及表达趋势  

Construction and expression trend of ceRNA network in human head and neck squamous cell carcinoma

作　　者：张岚[1] 刘法昱[2] ZHANG Lan;LIU Fa-yu(Department of Oral and Maxillofacial Surgery,Shool&Hospital of Stomatology,China Medical University,Shenyang 110002,China;Hospital Infection Management Office,Shool&Hospital of Stomatology,China Medical University,Shenyang 110002,China)

机构地区：[1]中国医科大学附属口腔医院院内感染管理办公室,辽宁沈阳110002 [2]中国医科大学附属口腔医院口腔颌面外科,辽宁沈阳110002

出　　处：《解剖科学进展》2020年第2期143-145,149,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金青年基金(81102058).

摘　　要：目的分别比较4例HNSCC患者癌和癌旁组织的环状RNA、微小RNA和mRNA芯片表达谱,构建hsacircRNA103580/hsa-miR-9-5P/PRDM15相关的ceRNA网络,探讨其表达趋势。方法利用芯片筛选HNSCC组织中差异表达的circRNA、miRNA和mRNA基因,根据miRwalk3.0在线软件预测的结果,构建HNSCC中的circRNA/miRNA/mRNA-相关的ceRNA网络。采用qRT-PCR方法检测HNSCC组织中hsacircRNA103580/hsamiR-9-5P/PRDM15基因的表达。结果芯片技术检测4例HNSCC患者癌和癌旁组织中差异表达的circRNA、miRNA及mRNA基因,获得显著差异的环状RNAs转录本311个,microRNAs转录本52个及circRNA433个。结合以上数据,构建了一个包含48种circRNAs,21种miRNAs和79种mRNAs的ceRNA网络并验证has-circRNA103580、has-miR-9-5p与PRDM15的基因表达水平与芯片中表达趋势一致。结论hsacircRNA103580/hsa-miR-9-5P/PRDM15轴可能是HNSCC预后和治疗新的靶点。Objective The expression profiles of circrnas,micrornas and mRNA microarrays in cancer and paracancer tissues of 4 head and neck squamous cell carcinoma(HNSCC)patients were compared,to construct the ceRNA network related to hsacircRNA103580/hsa-mir-9-5 p/PRDM15,and to explore the expression trend.Methods The microarray was used to screen the differentially expressed circRNA,miRNA and mRNA genes in HNSCC tissues,and the circRNA/miRNA/mra-related ceRNA network in HNSCC was constructed according to the results predicted by the online software miRwalk3.0.HsacircRNA103580/hsa-mir-9-5 p/PRDM15 gene expression in HNSCC tissues was detected by qrt-PCR.Results The differentially expressed circRNA,miRNA and mRNA genes in the cancer and paracancer tissues of 4 HNSCC patients were detected by microarray technology,and 311 circrnas,52 microRNAs and 433 circRNA transcripts with significant differences were obtained.A ceRNA network containing 48 circRNAs,21 miRNAs and 79 mRNAs was constructed,and the gene expression levels of has-circrna103580,has-mir-9-5 p and PRDM15 were verified to be consistent with the expression trend in the chip.Conclusion HsacircRNA103580/hsa-mir-9-5 p/PRDM15 axis is a new target for HNSCC prognosis and treatment.

关 键 词：乳颈部鳞癌 生物信息学分析 ceRNA 

分 类 号：R739.91[医药卫生—肿瘤]