老年结直肠癌患者肠道菌群变化与机体免疫炎症营养相关性  

The correlation between gut microbiota changes with immunity inflammation nutrition in elderly patients with colorectal cancer

作　　者：余莉[1] 李红[1] 王思平[1] YU Li;LI Hong;WANG Siping(Domestic Inpatient Department,Chinese PLA General Hospital,Beijing100853,China)

机构地区：[1]中国人民解放军总医院国宾二科,北京100853

出　　处：《中国微生态学杂志》2020年第4期404-409,414,共7页Chinese Journal of Microecology

基　　金：北京市科技计划——首都市民健康项目培育(Z151100003915124)。

摘　　要：目的探讨老年结直肠癌患者肠道菌群变化与机体免疫、炎症、营养指标的相关性,为结直肠癌的防治提供参考。方法纳入2016年1月至2018年6月在我院接受治疗的老年结直肠癌患者30例和同期健康查体的老年人群30例,收集粪便标本,采用FloraCheckTM高通量测序技术对粪便样本中所有细菌的16SrRNA V3-V4区进行DNA测序,序列通过MiSeq 16S宏基因组APP及QIIME软件,分析两组人群间属水平上显著差异性菌群。比较两组人群炎性指标(TNF-α、IL-6、IL-1、IL-12)、营养指标(总蛋白、白蛋白、前白蛋白、转铁蛋白)和免疫指标(CD3^+、CD4^+、CD8^+、NK)的差异。分析老年结直肠癌患者肠道菌群变化与营养、炎症、免疫的内在联系。结果老年结直肠癌组和老年健康组人群免疫指标CD3^+、CD4^+、CD8^+、NK和CD4^+/CD8^+比较差异无统计学意义;炎性指标老年结直肠癌组TNF-α含量较对照组明显增高(t=3.769,P=0.001),两组人群IL-1、IL-6、IL-12比较差异无统计学意义;营养指标老年结直肠癌组白蛋白、前白蛋白较对照组明显下降(t=-4.107,P=0.001;t=-4.366,P=0.001),两组人群总蛋白、转铁蛋白比较差异无统计学意义。老年结直肠癌组肠道差异性菌属变化,包括Eubacterium、Ruminococcaceae_UCG-002、Peptostreptococcus、Porphyromonas和部分炎症指标、营养指标、免疫指标有明显相关性。结论老年结直肠癌患者肠道菌群结构和功能异常,可能是导致患者机体免疫损伤、炎症反应、营养不佳的主要原因之一。有针对性地对肠道菌群结构进行优化,改善肠道菌群对宿主的影响,是一种帮助老年结直肠癌患者促进健康的策略。Objective To investigate the correlation between gut microbiota changes with immunity, inflammation and nutrition in elderly patients with colorectal cancer, and provide reference for the prevention and treatment of colorectal cancer. Methods Fecal specimens were collected from 30 elderly patients with colorectal cancer treated in our hospital from January 2016 to June 2018 and 30 elderly people who underwent health examination at the same time. DNA sequencing of 16 S rRNA V3-V4 region of all bacteria in fecal samples was performed by Flora CheckTM high-throughput sequencing technology. The sequence was sequenced by MiSeq 16 S macrogenome APP. And QIIME software was used to analyze the significant difference in genera level between the two groups. The differences of inflammatory index(TNF-a, IL-6, IL-1, IL-12), nutritional index(total protein, albumin, prealbumin, transferrin), immune index(CD3+, CD4+, CD8+, NK) between the two groups were compared. The relationship between gut microbiota changes with nutrition inflammation immunity in elderly patients with colorectal cancer were analyzed. Results There were no significant difference in the immune indices of CD3+, CD4+, CD8+, NK, CD4+/CD8+ between the elderly colorectal cancer group and the elderly healthy group;the content of TNF-alpha in the elderly colorectal cancer group was significantly higher than that in the control group(t=3.769,P=0.001);there were no significant difference in the nutritional indices of IL-1, IL-6 and IL-12 between the two groups. Albumin and prealbumin were significantly lower than those in the control group(t=-4.107,P=0.001;t=-4.366,P=0.001). There was no significant difference in total protein and transferrin between the two groups. There were significant correlations among intestinal differential bacteria in elderly patients with colorectal cancer, including Eubacterium, RuminococcaceaeUCG-002, Peptostreptococcus, Porphyromonas and some inflammatory, nutritional and immune indicators. Conclusion The abnormal structure and function of

关 键 词：结直肠癌 老年人 肠道菌群失衡 免疫应答 炎性指标 

分 类 号：R735.3[医药卫生—肿瘤]