液质饲料喂养制备酒精性心肌病小鼠模型  

Establishment of a mouse model of alcoholic cardiomyopathy with liquid feed

作　　者：廉虹[1] 刘立会 聂宇[1] LIAN Hong;LIU Lihui;NIE Yu(Chinese Academy of Medical Sciences,Peking Union Medical College,National Cardiovascular Center,Fuwai Hospital,State Key Laboratory of Cardiovascular Diseases,Beijing 100037,China)

机构地区：[1]中国医学科学院,北京协和医学院,国家心血管病中心,阜外医院,心血管疾病国家重点实验室,北京100037

出　　处：《中国比较医学杂志》2020年第5期40-46,共7页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金青年项目(81900343);北京市自然科学基金面上项目(7182140)。

摘　　要：目的利用液质饲料喂养的方法建立酒精性心肌病小鼠模型,为酒精性心肌病机制研究提供良好动物模型。方法利用含有酒精的液体饲料和不含酒精的对照饲料喂养16周龄C57小鼠(酒精组n=21;不含酒精组n=9),含有酒精的饲料酒精浓度由4.8%逐步升高至5.4%,共喂养8周,每周进行体重测量,监测死亡率,8周后进行心脏超声心动图检测以及病理学检测。结果酒精喂食组从第二周体重开始降低,一直持续到第八周体重较对照组均有明显的减轻;同时酒精组小鼠在酒精喂食期间死亡7只(占33.3%),而对照组没有死亡现象;心脏超声结果显示,酒精组小鼠较对照组心室前壁、后壁均显著变薄,心室内径增大,并且心脏射血分数和短轴缩短率显著变小;酒精组小鼠心脏/体重指数较对照组显著增大,同时HE染色结果也证实酒精组小鼠心脏明显增大。结论经过8周的酒精液质饲料喂养后,小鼠心脏呈现整体变大,左室壁变薄,心功能变差等酒精性心肌病的典型病理特征。因此,通过此方法能够成功制备酒精性心肌病小鼠模型。Objective A mouse model of alcoholic cardiomyopathy was established with liquid feed,which provided a good animal model to study alcoholic cardiomyopathy.Methods Sixteen-week-old C57BL/6 mice(n=21 alcohol group,n=9 alcohol-free group)were fed with liquid feed with or without alcohol.The alcohol concentration in the feed was gradually increased from 4.8%to 5.4%for 8 weeks,and weight and mortality were monitored.Echocardiography and pathology were performed after 8 weeks.Results The weights of the alcohol feeding group began to decrease by the second week.Compared with the control group,body weight was significantly reduced.In addition,seven mice(33.3%)died during the alcohol feeding period,whereas none of the mice in the control group died.The result of cardiac ultrasounds showed that the mice in the alcohol group had significantly thinner anterior and posterior ventricles,larger ventricular diameters,and the ejection fraction and shortening rate of the short-axis of the mice were significantly decreased.The heart/body mass indexes of mice in the alcohol group were significantly larger than those of the control group,and HE staining confirmed that the hearts of the mice in the alcohol group were significantly enlarged.Conclusions After 8 weeks of feeding with alcoholic liquid feed,the hearts of mice showed significantly larger and thinner left ventricular walls and cardiac dysfunction.Therefore,this method can be used to successfully establish a mouse model of alcoholic cardiomyopathy.

关 键 词：酒精性心肌病 小鼠模型 液质饲料 

分 类 号：R-33[医药卫生]