SMA小鼠中相关剪接因子表达与SMN2外显子7列入比率关系研究  

Expression and relationship between splicing factors and the full-length ratio of SMN2 in SMA mice

作　　者：杜利莉 马钰 柏文清 吴刘成 邵义祥 DU Lili;MA Yu;BAI Wenqing;WU Liucheng;SHAO Yixiang(Key Laboratory of Neuroregeneration,Nantong University,Nantong 226001,China;Laboratory Animals Center,Nantong University,Nantong 226001;Xinglin College,Nantong University,Nantong 226001)

机构地区：[1]南通大学神经再生重点实验室,江苏南通226001 [2]南通大学实验动物中心,江苏南通226001 [3]南通大学杏林学院,江苏南通226001

出　　处：《中国比较医学杂志》2020年第5期54-63,共10页Chinese Journal of Comparative Medicine

基　　金：江苏省研究生科研与实践创新计划项目(KYCX18-2415);南通市自然基金项目(JC2018090);南通大学大型仪器设备开放基金(KBJN1901)。

摘　　要：目的研究SMN2外显子7在SMA小鼠不同组织的列入,筛选与其相关的剪接因子。方法通过RT-PCR和非变性PAGE电泳分析在神经及非神经组织中SMN2外显子7列入比例,通过QPCR筛选分析剪接因子在大脑和脊髓与非神经组织中的mRNA水平。结果SMN2外显子7在大脑和脊髓组织中列入比例显著高于非神经组织,QPCR筛选显示Nova1/2在大脑和脊髓中的表达显著高于大部分非神经组织。通过差异分析显示,Srsf7/9及Nova2在SMAⅠ型小鼠脊髓中的表达显著高于对照小鼠。结论神经组织中,Srsf7/9和Nova1/2基因高表达与SMN2外显子7列入比例显著提高呈正相关,剪接因子SRSF7/9及NOVA1/2可能参与SMN2基因的剪接调控。Objective We examined the inclusion ratio of SMN2 exon 7 in different tissues from SMA mice and screened related splicing factors.Methods Ratios of SMN2 exon 7 inclusion in neural and non-neural tissues were analyzed by RT-PCR and non-denaturing PAGE electrophoresis.The expression levels of splicing factors in the brain,spinal cord,and non-neural tissues were analyzed by QPCR.Results The inclusion ratio of SMN2 exon 7 in brain and spinal cord tissues was significantly higher than that in non-neural tissues.The QPCR screening result showed that the expression of Nova1/2 was significantly higher in the brain and spinal cord than in most non-neural tissues.The difference analysis showed that the mRNA levels of Srsf7/9 and Nova2 in the spinal cord of SMA type I mice were significantly higher than those in control mice.Conclusions The high expression of Srsf7/9 and Nova1/2 is positively correlated with significant increases in the inclusion ratio of SMN2 exon 7 in neural tissues,indicating that SRSF7/9 and NOVA1/2 may be involved in the splicing regulation of SMN2.

关 键 词：SMA小鼠 剪接因子 表达 SMN2 外显子列入 

分 类 号：R-33[医药卫生]