四种有机溶剂对斑马鱼眼睛发育的影响  

Effects of four carrier solvents on eye development in zebrafish embryos

作　　者：邸亚男 朱丽英[2] 沈婕 钱雯 潘卫[2,3,4] DI Yanan;ZHU Liying;SHEN Jie;QIAN Wen;PAN Wei(Clinical Laboratory in Beifang Section of Peking University Third Hospital,Clinical Laboratory in Beijing Northern Hospital of Weaponry Industry,Bejing 100089,China;School of Clinical Laboratory Science in Guizhou Medical University,Guiyang 550025;Guizhou Prenatal Diagnosis Center,Affilated Hospttal of Guizhou Medical University,Guiyang 550025;Key Laboratory of Envionmental Pollution and Disease Monitoring of Ministry of Education,Guizhou Medical University,Guiyang 550025)

机构地区：[1]北京大学第三医院北方院区检验科,兵器工业北京北方医院检验科,北京100089 [2]贵州医科大学检验医学院,贵阳550025 [3]贵州医科大学附属医院贵州省产前诊断中心,贵阳550025 [4]贵州医科大学环境污染与疾病监控教育部重点实验室,贵阳550025

出　　处：《中国比较医学杂志》2020年第5期75-80,共6页Chinese Journal of Comparative Medicine

基　　金：贵州省科技支撑计划资助项目(黔科合支撑[2017]2970);贵族省域内一流学科建设项目:黔教科研发2017[85]号。

摘　　要：目的评估乙醇、异丙醇、丙酮和二甲基亚砜四种有机溶剂对斑马鱼胚胎发育,尤其是眼睛发育的影响,为其作为溶剂在斑马鱼眼睛疾病模型研究中提供参考。方法将发育至12 hpf斑马鱼胚胎暴露于0.5%乙醇、1%异丙醇、1.5%丙酮和2.5%二甲基亚砜至72 hpf,观察其存活率、致畸率及眼睛发育状况。结果对照组和乙醇、异丙醇、丙酮、DMSO暴露组存活率分别为96%和93%、90%、90%、86%。对照组斑马鱼幼鱼形态正常,而有机溶剂暴露组则出现了不同程度形态变化如心包水肿、出血、卵黄缺损和身体弯曲等。其中,以DMSO致畸作用最严重,乙醇的致畸作用最弱。方差分析显示,斑马鱼幼鱼眼睛长度/身体长度比值在有机溶剂暴露组和对照组之间没有显著差异;但是DMSO暴露组斑马鱼幼鱼眼睛面积/身体面积比值显著低于其他组。另外与对照组相比,四种有机溶剂暴露组的视网膜分层均为正常,乙醇、异丙醇、丙酮暴露组视锥细胞、视杆细胞和视网膜神经元细胞信号正常,但DMSO暴露组各细胞信号均较弱。结论因此,在以斑马鱼为模型动物进行眼睛疾病的相关研究时,我们应谨慎选择有机溶剂作为药物或其他化学受试品的溶解载体,并且应该进行预实验来确定其最佳使用浓度。Objective To study the effects of four organic solvents,including methanol,isopropanol,acetone,and dimethyl sulfoxide(DMSO),on the development of zebrafish embryos,especially eye development,and provide clues for their use in zebrafish studies.Methods Zebrafish embryos(12 hours post-fertilization)were exposed to 0.5%ethanol,1%isopropanol,1.5%acetone,and 2.5%DMSO for 60 hours.The survival rate,eye size,gross morphological changes,and retinal histological features were observed.Results The survival rates of the control group and the groups exposed to ethanol,isopropanol,acetone,and DMSO were 96%,93%,90%,90%,and 86%,respectively.The morphology of the zebrafish was normal in the control group,whereas there were morphological changes,such as pericardial edema,hemorrhage,yolk defect,and body bending,in the solvent-treated groups.Specifically,DMSO induced the most severe changes,and ethanol the least.Variance analysis showed that there was no significant difference in the ratio of eye length to body length between the organic solvent-treated groups and the control group,but the ratio of the eye area to body area in the group treated with DMSO was significantly lower than that in other groups.In addition,compared with the control group,the retina stratification in the group treated with four organic solvents was normal.The signals of cone cells,rod cells,and retinal neurons in the ethanol,isopropanol,and acetone exposed groups were normal,but the signals of each cell were weak in the group treated with DMSO.Conclusions Therefore,we should carefully select the solvent carrier for zebrafish studies and perform preliminary experiments to determine the safest concentration.

关 键 词：有机溶剂 使用浓度 斑马鱼 眼睛发育 

分 类 号：R-33[医药卫生]