舒血宁注射液对急性脑梗死大鼠脑组织的保护作用及其机制  

Protective effect of Shuxuening Injection on brain tissue of rats with acute cerebral infarction and its mechanism

作　　者：焦光美[1] 单海雷 张晓璇[1] 赵亮[1] 窦志杰[1] 康玲伶[1] 马征[1] 孙艳军[1] 杨宁[1] JIAO Guangmei;SHAN Hailei;ZHANG Xiaoxuan;ZHAO Liang;DOU Zhijie;KANG Lingling;MA Zheng;SUN Yanjun;YANG Ning(Department of Neurology, Affiliated Hospital, Chengde Medical College, Chengde 067000, China)

机构地区：[1]承德医学院附属医院神经内科,河北承德067000

出　　处：《吉林大学学报（医学版）》2020年第3期557-562,共6页Journal of Jilin University：Medicine Edition

基　　金：河北省卫健委医学科学项目资助课题(20181160)。

摘　　要：目的:探讨舒血宁注射液对急性脑梗死大鼠脑组织的保护作用,并阐明其作用机制。方法:50只大鼠随机分为对照组、假手术组、模型组、尼莫地平组和舒血宁注射液组(n=10)。模型组、尼莫地平组和舒血宁注射液组采用颈内动脉线栓法建立急性脑梗死大鼠模型。假手术组大鼠分离颈总动脉、颈外动脉和颈内动脉后,只结扎颈外动脉;对照组大鼠不作手术处理。舒血宁注射液组大鼠造模成功后给予舒血宁注射液,尼莫地平组大鼠造模后给予尼莫地平,对照组、模型组和假手术组大鼠给予等体积生理盐水。检测各组大鼠神经功能缺损评分、脑组织含水量和相对脑梗死面积,HE染色观察各组大鼠脑组织形态表现,免疫组织化学染色检测各组大鼠脑组织中生长分化因子15(GDF-15)和C反应蛋白(CRP)表达水平,ELISA法检测各组大鼠脑组织中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)和白细胞介素1β(IL-1β)水平。结果:对照组和假手术组大鼠脑皮层结构完整,细胞排列整齐;模型组大鼠神经细胞胞浆和胞核皱缩,神经细胞与毛细管周围间质疏松;舒血宁注射液组大鼠脑皮质神经细胞、神经胶质细胞肿胀程度及间质疏松程度均减轻,病理组织形态表现与尼莫地平组相近。与对照组和假手术组比较,模型组大鼠脑组织含水量升高(P<0.05),脑组织中CRP表达水平和TNF-α、IL-6和IL-1β水平升高(P<0.05),GDF-15表达水平明显降低(P<0.05)。与模型组比较,尼莫地平组和舒血宁注射液组大鼠神经功能缺损评分降低(P<0.05),脑组织含水量明显降低(P<0.05),相对脑梗死面积减小(P<0.05),脑组织中CRP表达水平和TNF-α、IL-6及IL-1β水平均明显降低(P<0.05),GDF-15表达水平明显升高(P<0.05)。与尼莫地平组比较,舒血宁组大鼠神经功能缺损评分,脑组织含水量,相对脑梗死面积,脑组织中CRP和GDF-15表达水平及TNF-α、IL-6、IL-1β水平比较�Objective:To investigate the protective effect of Shuxuening Injection on the brain tissue of the rats with acute cerebral infarction,and to elucidate its mechanism.Methods:Fifty rats were randomly divided into control group,sham operation group,model group,nimodipine group and Shuxuening Injection group(n=10).The rat models of acute cerebral infarction were made by internal carotid artery suture method in model group,nimodipine group and Shuxuening Injection group.The common carotid arteries,the external carotid arteries and the internal carotid arteries of the rats in sham operation group were separated,and only the external carotid arteries were ligated;the rats in control group were not treated with operation;the rats in Shuxuening Injection group were given Shuxuening Injection,the rats in nimodipine group were given nimodipine,and the rats in control group,model group and sham operation group were given normal saline at the same volume.The score of neurological impairment,water contents in brain tissue and relative cerebral infarction areas of the rats in various groups were measured.HE staining was used to observe the morphology of brain tissue of the rats in various groups,and immumohistochemical staining was used to detect the expression levels of growth differentiation factor-15(GDF-15)and C-reactive protein(CRP)in brain tissue of the rats in various groups.ELISA method was used to detect the levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-1β(IL-1β)in brain tissue of the rats in various groups.Results:The cortical structures of cortex of the rats in control group and sham operation group were complete and the cells were arranged neatly;the cytoplasm and nucleus of the nerve cells of the rats in model group were wrinkled,and the interstitium between nerve cells and capillaries were loose;the swelling degrees of cerebral cortical nerve cells and glial cells and the loose degrees of stroma of the rats in Shuxuening Injection group were reduced,and the histopathological manif

关 键 词：脑梗死 舒血宁注射液 生长分化因子15 C反应蛋白 

分 类 号：R743.33[医药卫生—神经病学与精神病学]