Cu污染对弹尾目跳虫超显微结构影响的初步研究  

Ultrastructure of Folsomia Candida Cell under Soil Copper Contamination

作　　者：李晓勇 柯欣[2,3] 廖剑宇 LI Xiao-yong;KE Xin;LIAO Jian-yu(Institute of Urban and Environment,Hunan University of Technology,Zhuzhou 412007,China;Key Laboratory of Soil Environment and Pollution Remediation,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China;Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China)

机构地区：[1]湖南工业大学城市与环境学院,湖南株洲412007 [2]中国科学院土壤环境与污染修复重点实验室南京土壤研究所,江苏南京210008 [3]中国科学院上海生命科学院植物生理生态研究所,上海200032

出　　处：《土壤通报》2020年第1期221-226,共6页Chinese Journal of Soil Science

基　　金：湖南省教育厅一般项目(17C0464)资助。

摘　　要：土壤重金属生态毒理学研究已成为当今生态与环境科学研究的热点。土壤弹尾目跳虫作为土壤模式生物,在土壤生态毒理学和生态环境评估研究中已被广泛应用。研究通过跳虫(F.candida)的标准土壤实验,利用电子显微镜技术,初步观察到暴露于土壤中高Cu浓度(1000,3000μg g-1)下的跳虫细胞微绒毛变形、部分线粒体扩大变形并出现囊泡化现象,这可能会导致细胞组织新陈代谢功能减弱进而影响跳虫的生理行为;同时,在高Cu暴露浓度下跳虫细胞内A型颗粒物大量存在,且其环边逐渐减少、变得模糊,这说明Cu的存在可能使A型颗粒物的结构和成分发生变化并变为储存Cu的重要细胞器。Ecotoxicity of heavy metals in soil has become a research hotspot in recent years.As an important component of soil ecosystem,Folsomia candida has been widely used in soil ecotoxicology and ecological environment assessment.A standard soil experiments of F.candida,Collembola were carried out by using transmission electron microscopy.Under high concentrations of Cu(1000 and 3000μg g-1),microvilli of the F.candida midgut cells appeared be out of shape,and part of mitochondria was amplified and became vesicles,which may weaken the metabolism of the cells and influence the physiological behavior of F.candida.A lot of A-type granules occurred throughout the cell of F.candida,and their circular edge reduced and became vague gradually under high concentrations of Cu.This indicated that the alteration in the structure and constituent of A-type granules may be an important organelle to store Cu.

关 键 词：超显微结构 白符跳(Folsomia candida) 急性毒性 铜 

分 类 号：X180.4425[环境科学与工程—环境科学]