灰树花多糖体外抑菌及抗氧化活性研究  

Study on Antibacterial in Vitro and Antioxidant Activity of Grifola Frondosa Polysaccharide

作　　者：付佳乐[1] 耿直[1] FU Jiale;GENG Zhi(Shaanxi Institute of International Trade Commerce,Xi’an 712046,China)

机构地区：[1]陕西国际商贸学院,西安712046

出　　处：《中国现代应用药学》2020年第8期945-948,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的研究灰树花多糖的抑菌及抗氧化活性。方法采用紫外分光光度法,以D-无水葡萄糖为对照品,测定灰树花提取物中多糖含量;采用纸片扩散法,以头孢哌酮为阳性对照,测量不同浓度灰树花多糖提取物金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌的抑菌圈直径;采用DPPH法和T-AOC试剂盒法,以清除率为指标,研究灰树花多糖的抗氧化活性。结果灰树花提取物中多糖含量为53.49%;灰树花提取物对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌均有一定的抑菌作用,抑菌圈直径分别为13,11,10mm;灰树花多糖浓度为0.05~10mg·mL^-1时,对DPPH自由基具有一定的清除能力,当浓度高于0.5 mg·mL^-1时,开始显示出总抗氧化能力。结论灰树花多糖具有一定的抑菌和抗氧化能力。OBJECTIVE To study the antibacterial effect and antioxidant activity of Grifola frondosa polysaccharide.METHODS UV spectrophotometry was used to determine the content of polysaccharide,D-anhydroglucose was used as a standard.The antibacterial effect produced by Kirby-Baue method with the bacteria of Staphylococcus aureus,Escherichia coli and Bacillus subtilis.Took the scavenging capacity as index to estimate the endogenous antioxidative activity of DPPH and T-AOC.RESULTS The total polysaccharides in Grifola frondosa extract was 53.82%.Grifola frondosa polysaccharide had a good antibacterial effect on Staphylococcus aureus,Escherichia coli and Bacillus subtilis,the diameter of antibacterial were 13,11,10 mm.With the concentration between 0.05~10 mg·mL^-1,Grifola frondosa polysaccharide had a good scavenging activity by DPPH,when the concentration above 0.5 mg·mL^-1,showed the total antioxidant activity.CONCLUSION The polysaccharides in Grifola frondosa has certain antibacterial effect and antioxidant activity.

关 键 词：灰树花 多糖 抑菌 抗氧化 

分 类 号：R285.5[医药卫生—中药学]