二氯乙酸盐对人胰腺癌细胞增殖及迁移的作用  

Dichloroacetate effects on proliferation and migration of pancreatic cancer cells

作　　者：张秀 周守兵 姜洁 周磊磊[1] 岳顺[1] Zhang Xiu;Zhou Shou-bing;Jiang Jie;Zhou Lei-lei;Yue Shun(Department of Oncology,The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University,Hai'an 223001;Department of Oncology,The First Affiliated Hospital of USTCWest District,Hefei 230031)

机构地区：[1]南京医科大学附属淮安第一医院,淮安223001 [2]中国科学技术大学附属第一医院西区,合肥230031

出　　处：《广西医科大学学报》2020年第5期872-877,共6页Journal of Guangxi Medical University

摘　　要：目的:探讨二氯乙酸盐(DCA)对人胰腺癌细胞株Panc-1增殖、迁移的影响及小分子化合物968对DCA抑制胰腺癌细胞株Panc-1增殖的协同作用。方法:采用MTT法检测DCA、DCA与968协同对Panc-1细胞株增殖的影响;利用划痕实验观察DCA对Panc-1细胞株迁移的影响;通过逆转录PCR(RT-PCR)检测E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)的mRNA表达水平。结果:相比于对照组,经DCA处理后,Panc-1细胞株活性显著降低(P<0.05),Panc-1细胞株迁移受抑制,Ecadherin的mRNA表达水平升高、N-cadherin mRNA表达水平下降(P<0.05),且968与DCA具有协同杀伤胰腺癌细胞的作用(P<0.05)。结论:DCA、DCA联合968可能成为胰腺癌治疗的一种新策略,抑制胰腺癌细胞的糖代谢有望成为胰腺癌靶向代谢治疗的新方法。Objective:To investigate the effects of DCA on proliferation and migration of human pancreatic cancer Panc-1 cells and detect synergistic effect of DCA combined with micromolecular compound 968 on proliferation of pancreatic cancer cells.Methods:MTT assay was applied to assess the cell proliferation in DCA treating group and DCA collaborated with 968 treating group.Scratch test was used to examine influence of DCA on migration of Panc-1 cells.The levels of mRNAs of E-cadherin and N-cadherin were analyzed by RT-PCR.Results:DCA can attentuate cell viability,inhibit migration,increase the level of mRNAs of E-cadherin and decrease the level of mRNAs of N-cadherin in Panc-1 cells.Conclusion:DCA,DCA combined with 968 could be the new method for pancreatic cancer treatment.Inhibiting glucose metabolism could be a novel way for metabolic therapy of pancreatic cancer.

关 键 词：胰腺癌 二氯乙酸盐 968 增殖 迁移 

分 类 号：R735.9[医药卫生—肿瘤]