钙敏感受体在大鼠脑缺血再灌注损伤时细胞凋亡中的作用  

Role of Calcium Sensing Receptor on Cerebral Ischemia-reperfusion Injury in Rats

作　　者：李凤晓[1] 史雪莹 初向华[1] 卢晓东[1] 董加花[1] 于良健 张宁[2] LI Feng-xiao;SHI Xue-ying;CHU Xiang-hua;LU Xiao-dong;DONG Jia-hua;YU Liang-jian;ZHANG Ning(Department of Pharmacy,Affiliated Hospital of Qingdao University,Qingdao,Shandong,266003,China;Department of Anesthesiology,Qingdao Municipal Hospital,Qingdao,Shandong,266011,China)

机构地区：[1]青岛大学附属医院药学部,山东青岛266003 [2]青岛市市立医院麻醉科,山东青岛266011

出　　处：《现代生物医学进展》2020年第6期1043-1046,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81771415)。

摘　　要：目的:评价钙敏感受体在大鼠脑缺血再灌注损伤时细胞凋亡中的作用。方法:健康成年雄性Wistar大鼠60只,体重250~300 g,采用随机数字表法分为3组(n=20):假手术组(S组)、脑缺血再灌注组(I/R组)和钙敏感受体拮抗剂组(N组)。I/R组和N组采用线栓法经左侧颈外-颈内动脉插线制备大鼠脑缺血再灌注损伤模型,于脑缺血前10 min尾静脉注射等容量二甲基亚砜和钙敏感受体拮抗剂NPS-896361 mg/kg。于再灌注24 h时行神经功能评分,随后处死大鼠取脑组织,测定MDA含量和SOD活性,采用TUNEL法观察神经细胞凋亡情况,计算神经细胞凋亡指数,免疫组化法检测Caspase-3阳性细胞的表达,Western blot法检测Caspase-3蛋白的表达。结果:I/R组和N组MDA含量、神经细胞凋亡指数、Caspase-3阳性细胞和Caspase-3蛋白表达水平高于S组,神经功能评分和SOD活性低于S组,差异有统计学意义(P<0.05);N组MDA含量、神经细胞凋亡指数、Caspase-3阳性细胞和Caspase-3蛋白表达水平低于I/R组,神经功能评分和SOD活性高于I/R组,差异有统计学意义(P<0.05)。结论:钙敏感受体参与大鼠脑缺血再灌注损伤和细胞凋亡的发生。Objective: To investigate the effect of calcium sensing receptor on cerebral ischemia-reperfusion(I/R)in rats. Methods:Sixty male Wistar rats, weighing 250-300 g, were randomly divided into three groups(n=20 each)using a random number table: sham operation group(group S), group I/R and calcium-sensing receptor antagonists group(group N). Animal models of middle cerebral artery occlusion/reperfusion in the later 2 groups were established by a filament method in the left external-internal carotid artery. Rats were injected with 1 mg/kg NPS-89636 in the calcium-sensing receptor antagonists group and the equal volume of dimethyl sulfoxide 10 minutes before the cerebral ischemia. Neurological function was assessed and scored at 24 h of reperfusion. The rats were sacrificed and then the brains were removed for determination of MDA content, SOD activity, apoptotic cell death was assessed by TUNEL staining,and the expression of Caspase-3 by immunohistochemical and Western blot. Results: The content of MDA, neuronal apoptosis index,expression levels of Caspase-3 positive cells and Caspase-3 protein in group I/R and N were higher than those in group S, and neurological function score and SOD activity were lower than those in group S(P<0.05);The content of MDA, neuronal apoptosis index,expression levels of Caspase-3 positive cells and Caspase-3 protein in group N were lower than those in group I/R, and neurological function score and SOD activity were higher than those in group I/R(P<0.05). Conclusion: Calcium-sensing receptor is involved in cerebral ischemia-reperfusion injury and cell apoptosis.

关 键 词：受体 钙敏感 再灌注损伤 脑 细胞凋亡 

分 类 号：R-33[医药卫生] R743