Evolutionary and functional analyses of the interaction between the Bombyx mori inhibitor of apoptosis(IAP)and nucleopolyhedrovirus IAPs  

作　　者：Peng Chen Tao-Tao Kang Xi-Yan Bao Zhan-Qi Dong Yan Zhu Wen-Fu Xiao Min-Hui Pan Cheng Lu 

机构地区：[1]State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing,China [2]Key Laboratory of Sericultural Biology and Genetic Breeding,Ministry of Agriculture,Southwest University,Chongqing,China

出　　处：《Insect Science》2020年第3期463-474,共12页昆虫科学（英文版）

基　　金：This work was funded by National Natural Science Foundation of China(No.31602009,31572466 and 31472152);Fundamental Research Funds for the Central Universities(SWU116066 and XDJK2018C009),China Agriculture Research System(CARS-18),China Postdoctoral Science Foundation(2015M582502);Chongqing Postdoctoral Science Foundation(Xm2015121).

摘　　要：As an important insect immune response,apoptosis plays a critical role in the interaction between baculoviruses and insect hosts.Previous reports have identified inhibitor of apoptosis(IAP)proteins in both insects and baculoviruses,but the relationship between these proteins is still not clearly understood.Here,we found that insect IAP proteins were clustered with baculovirus IAP3,suggesting that the baculovirus iap3 gene might be derived from the Lepidoptera or Diptera.We demonstrated that Bombyx mori inhibitor of apoptosis(Bmiap)gene had an inhibitory effect on apoptosis in silkworm cells.Further analysis of the effects of Bmiap genes on the proliferation of B.mori nucleopolyhedrovirus(BmNPV)showed that both the Bmiap and BmNPV iap genes increased BmNPV proliferation after BmNPV infected silkworm cells.Our results also indicated that BmNPV IAP1 and IAP2 directly interacted with BmIAP in silkworm cells,implying that the Bmiap gene might be hijacked by BmNPV iap genes during BmNPV infection.Taken together,our results provide important insights into the functional relationships of iap genes,and improve our knowledge of apoptosis in baculoviruses and insect hosts.

关 键 词：BACULOVIRUS baculovirus-host interaction BMNPV Bombyx mori evolution inhibitor of apoptosis 

分 类 号：Q96[生物学—昆虫学]