分析血清IL-34水平与幽门螺杆菌感染相关性胃疾病的关系  

Analysis of the relationship between serum IL-34 level and Helicobacter pylori related gastric diseases

作　　者：周红菊 杨扬[1] 向瑜[1] ZHOU Hongju;YANG Yang;XIANG Yu(Department of Clinical Laboratory,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第一医院检验科,重庆400016

出　　处：《国际检验医学杂志》2020年第11期1333-1336,共4页International Journal of Laboratory Medicine

基　　金：重庆市科学技术委员会基础研究与前沿探索基金项目(cstc2018jcyjAX0748)。

摘　　要：目的检测胃癌、慢性胃炎患者血清中白细胞介素-34(IL-34)水平,探讨IL-34在幽门螺杆菌感染相关性胃疾病中的表达情况,以确定IL-34能否作为幽门螺杆菌感染相关性胃疾病的血清学标志物。方法从270例患有胃部疾病的住院患者中筛选出39例胃癌患者和21例慢性胃炎患者,采用酶联免疫吸附试验测定患者血清中IL-34水平,比较胃癌组、慢性胃炎组与40例体检健康者IL-34水平的表达差异。应用幽门螺杆菌抗体检测试剂盒检测上述患者和健康对照者幽门螺杆菌的感染情况。分析胃癌组和慢性胃炎组中IL-34与甲胎蛋白(AFP)、癌胚抗原(CEA)、糖类抗原19-9(CA19-9)的相关性。结果胃癌组IL-34的表达水平为6.57(3.86~9.71)pg/mL,慢性胃炎组IL-34的表达水平为12.71(2.89~19.42)pg/mL,与健康对照组比较,血清IL-34水平均升高,差异具有统计学意义(U=465,P<0.01;U=203,P<0.01)。39例胃癌患者中,幽门螺杆菌阳性12例(30.77%),21例慢性胃炎组中,幽门螺杆菌阳性5例(20.81%)。在胃癌患者中,幽门螺杆菌阴性组IL-34的表达水平为7.29(5.07~9.71)pg/mL,与健康对照组比较,有所升高,差异具有统计学意义(U=294,P<0.01)。在慢性胃炎患者中,幽门螺杆菌阴性组IL-34的表达水平为13.60(2.80~19.41)pg/mL,与健康对照组比较,有所升高,差异具有统计学意义(U=157,P<0.05)。结论血清IL-34水平在胃癌、慢性胃炎中表达增高,可以作为幽门螺杆菌感染阴性的胃癌、慢性胃炎的血清学标志物。Objective To detect the level of interleukin-34(IL-34)in the serum of patients with gastric cancer and chronic gastritis,and to explore the expression of IL-34 in Helicobacter pylori infection related gastric diseases,so as to determine whether IL-34 can be used as a serological marker of Helicobacter pylori infection related gastric diseases.Methods A total of 39 patients with gastric cancer and 21 patients with chronic gastritis were selected from 270 patients with gastric diseases.The level of IL-34 in serum of patients was measured by enzyme-linked immunosorbent assay,and the expression difference of IL-34 in gastric cancer group,chronic gastritis group and healthy control group was compared.Helicobacter pylori antibody detection kit was used to detect the infection of Helicobacter pylori in the above patients and healthy controls.The correlation between IL-34 and alpha-fetoprotein(AFP),carcinoembryonic antigen(CEA),and glycoprotein antigen(CA19-9)in gastric cancer group and chronic gastritis group was analyzed.Results The expression level of IL-34 in gastric cancer group was 6.57(3.86-9.71)pg/mL,and the expression level of IL-34 in chronic gastritis group was 12.71(2.89-19.42)pg/mL.Compared with healthy control group,the IL-34 level increased,and the difference was statistically significant(U=465,P<0.01;U=203,P<0.01).Of the 39 patients with gastric cancer,12 were positive for Helicobacter pylori infection(positive rate was 30.77%),and 5 were positive for Helicobacter pylori infection in 21 chronic gastritis groups(positive rate was 20.81%).In gastric cancer patients,the expression level of IL-34 in the Helicobacter pylori infection group was 7.29(5.07-9.71)pg/mL,which was increased compared with the healthy control group,and the difference was statistically significant(U=294,P<0.01).In patients with chronic gastritis,the expression level of IL-34 in the Helicobacter pylori negative group was 13.60(2.80-19.41)pg/mL,which was increased compared with the healthy control group,and the difference was statisticall

关 键 词：白细胞介素-34 胃癌 慢性胃炎 幽门螺杆菌 

分 类 号：R573[医药卫生—消化系统]