水杨酸诱导紫楠对炭疽病的抗性  

Resistance of Phoebe sheareri to anthracnose induced by salicylic acid

作　　者：侯盼盼 陈安良 费莉玢 马良进 HOU Panpan;CHEN Anliang;FEI Libin;MA Liangjin(National Joint Local Engineering Laboratory of Biopesticide High-efficient Preparation,School of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]浙江农林大学林业与生物技术学院生物农药高效制备技术国家地方联合工程实验室,浙江杭州311300

出　　处：《浙江农林大学学报》2020年第3期605-610,共6页Journal of Zhejiang A&F University

基　　金：国家林业局引进国际先进林业科学技术项目(2015-4-40)。

摘　　要：【目的】探究水杨酸(SA)诱导紫楠Phoebe sheareri对炭疽病的抗性。【方法】以2年生紫楠为材料,用0、50、100、200、500 mg·L^−1质量浓度SA对紫楠进行喷雾处理,以无菌水喷洒不接种为对照(ck)。喷雾后选择不同时间采集紫楠叶片,采用离体刺伤接种法测定对炭疽病病斑的抑制作用,并测定了紫楠叶片中可溶性蛋白质、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的变化。【结果】不同质量浓度SA处理的紫楠叶片均对炭疽病病斑有抑制作用,100 mg·L^−1 SA处理对病斑抑制率达64.28%,在SA喷雾的同时接种炭疽病孢子悬浮液后,叶片的可溶性蛋白质、SOD、CAT和POD在1~7 d内升高,达到峰值时可溶性蛋白质为ck的2.57倍,SOD活性为ck的1.21倍,CAT活性为ck的2.04倍,POD活性为ck的1.46倍,7 d后呈下降趋势,15 d内各生理指标均显著高于ck。【结论】SA可诱导紫楠叶片可溶性蛋白质、SOD、CAT和POD升高,对炭疽病产生抗病性。[Objective]The objective is to investigate the resistance of Phoebe sheareri to anthracnose induced by salicylic acid(SA).[Method]The 2-year old P.sheareri was used as material,spayed with 100,200,500 mg·L^−1 SA.After 5 days,the leaves were collected and in vitro puncture inoculation method was used to determine the inhibition of anthracnose lesions,changes in soluble protein,superoxide dismutase(SOD),catalase(CAT),and peroxidase(POD)in the leaves.[Result]Leaves treated with different concentrations of SA could inhibit the anthracnose lesions,and the lesion inhibition rate with 100 mg·L^−1 SA treatment reached 64.28%.After SA spray and inoculation with anthracnose,soluble protein,SOD,CAT and POD in leaves increased in 1−7 days,and decreased after 7 days.At the peak,the soluble protein was 2.57 times of ck,SOD was 1.21 times of ck,CAT was 2.04 times of ck,and POD was 1.46 times of ck.All physiological indexes were higher than those of ck within 15 days.[Conclusion]SA can induce the increase of soluble protein,SOD,CAT and POD of P.sheareri and produce disease resistance.

关 键 词：森林保护学 水杨酸 紫楠 胶孢炭疽菌 抗性诱导 

分 类 号：S763[农业科学—森林保护学]