利用转录组信息揭示刺芹侧耳单核和双核菌丝中小RNA的表达差异  

Transcriptome Sequencing Reveals Small RNAs Expression Differences in Monokaryotic and Dikaryotic Mycelia of Pleurotus eryngii

作　　者：尚俊军 汪滢[1] 龚明[1] 杨瑞恒[1] 唐利华[1] 万佳宁[1] 李焱 茅文俊[1] 本田与一 鲍大鹏[1] 李燕[1] SHANG Junjun;WANG Ying;GONG Ming;YANG Ruiheng;TANG Lihua;WAN Jianing;LI Yan;MAO Wenjun;HONDA Yoichi;BAO Dapeng;LI Yan(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources Utilization(South),Ministry of Agriculture and Rural Affairs P.R.China,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungi Processing,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;Laboratory of Forest Biochemistry,Division of Environmental Science and Technology,Graduate School of Agriculture,Kyoto University,Kyoto 606-8502,Japan)

机构地区：[1]上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海201403 [2]日本京都大学农业研究生院环境科学与技术系森林生物化学实验室,日本京都606-8502

出　　处：《食用菌学报》2020年第2期17-23,共7页Acta Edulis Fungi

基　　金：上海市科技兴农重点攻关项目[沪农科攻字(2015)第6-2-1号];上海食用菌工程技术研究中心(19DZ2281200);海市农业科学院2020年度农业科技创新支撑领域研究专项。

摘　　要：利用Illumina HiSeq 2500高通量测序平台,对刺芹侧耳(Pleurotus eryngii)双核菌株‘杏韩’及其原生质体单核化菌株‘181’和‘183’菌丝的小RNA文库进行测序分析。将测序获得的高质量reads与Rfam数据库和刺芹侧耳基因组测序数据进行比对,发现来源于rRNA、tRNA、snRNA和snoRNA等非编码RNA的reads占47.4%,来源于mRNA降解的reads占15.5%。将剩余的37.1%reads与miRBase数据库比对,发现946条小RNA匹配数据库中其他物种的成熟miRNA。进一步对这些小RNA在单核菌株和双核菌株中的表达量进行比较,发现双核菌株中高表达量的小RNA比单核菌株中多19.4%。单核和双核菌株差异表达小RNA分析结果显示,单核菌株中只有39条小RNA表达量上调,而双核菌株中有196条小RNA表达量上调。The Illumina HiSeq 2500 high-throughput sequencing platform was used to sequence and analyze mycelial small RNA libraries of Pleurotus eryngii dikaryotic strain ‘Xinghan’ and its protoplast-derived monokaryotic strains ‘181’ and ‘183’. High-quality reads obtained by sequencing were compared with the Rfam database and the P. eryngii genome sequence. The results showed that 47.4% of the reads were derived from non-coding RNAs such as rRNAs, tRNAs, snRNAs, and snoRNAs, while 15.5% of the reads were derived from mRNA degradation. The remaining reads were compared with the miRBase database, and 946 small RNAs were found to match mature miRNAs of other species in the database. The expression of these small RNAs was further compared in monokaryotic and dikaryotic strains. Proportion of high-expression small RNAs was 19.4% greater in dikaryotic strain ‘Xinghan’ than that in monokaryotic strains. There were only 39 small RNAs up-regulated in ‘181’ and ‘183’, whereas 196 small RNAs were up-regulated in the dikaryotic strain.

关 键 词：刺芹侧耳 转录组测序 小RNA 

分 类 号：S646.14[农业科学—蔬菜学]