利用高速逆流色谱技术制备灵芝酮三醇  

Preparative Isolation of Ganodermanontriol from Ganoderma lucidum by High-speed Countercurrent Chromatography

作　　者：岳亚文 田振[4] 张劲松[1] 王晨光 韩伟 唐庆九[1] 冯娜[1] 周帅[1] YUE Yawen;TIAN Zhen;ZHANG Jingsong;WANG Chenguang;HAN Wei;TANG Qingjiu;FENG Na;ZHOU Shuai(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungal Processing,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;Engineering Research Centre of Pharmaceutical Process Chemistry,Ministry of Education,East China University of Science and Technology,Shanghai 200237,China;Shanghai Baixin Bio-Tech Co.,Ltd.,Shanghai 201403,China;Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海市农业科学院食用菌研究所,国家食用菌工程技术研究中心,农业农村部南方食用菌资源利用重点实验室,上海市农业遗传育种重点开放实验室,上海201403 [2]华东理工大学药学院制药工程与过程化学教育部工程研究中心,上海200237 [3]上海百信生物科技有限公司,上海201403 [4]上海海洋大学,上海201306

出　　处：《食用菌学报》2020年第2期77-83,共7页Acta Edulis Fungi

基　　金：上海市科学技术委员会科研计划项目(17391903300)。

摘　　要：以沪农灵芝(Ganoderma lucidum)1号子实体为原料,经过87%乙醇提取、D101大孔树脂富集、乙醇梯度洗脱获得弱极性三萜组分。采用高速逆流色谱技术,以正己烷∶乙酸乙酯∶甲醇∶水(V∶V∶V∶V=5∶6∶5∶5)为溶剂体系,对该三萜组分中灵芝酮三醇的制备工艺进行研究,在转速900 r·min^-1、流速3 mL·min^-1条件下,一次上样400 mg灵芝弱极性三萜组分,可得到纯度为91.6%的灵芝酮三醇26.8 mg。Ganodermanontriol was purified from fruiting bodies of Ganoderma lucidum(Hunong No.1) following a series of steps, including extraction by 87% ethanol, condensation by macroporous adsorption resin D101, gradient elution by ethanol to obtain the weak polar triterpenes fraction(WTF), and finally preparation of the target compound from WTF by high-speed countercurrent chromatography(HSCCC). For HSCCC, the solvent system was determined to be a mixture of N-hexane,ethyl acetate, methanol and water(V∶V∶V∶V=5∶6∶5∶5);the separation condition was set as rotation at 900 rpm and flow rate of 3.0 mL·min^-1. Using the above mentioned HSCCC condition, a single loading amount of 400 mg WTF yielded 26.8 mg ganodermanontriol with a purity of 91.6%.

关 键 词：灵芝子实体 弱极性三萜 大孔树脂 高速逆流色谱 

分 类 号：R284[医药卫生—中药学]