我国部分地区采供血机构发现HIV-1感染者的毒株基因亚型分析  

Analysis of HIV-1 subtypes in infected people detected from blood stations in some regions of China

作　　者：段蕾静 张志敏 蒋岩[1] 狄宇 徐晴晴 潘品良[1] 邱茂锋[1] DUAN Leijing;ZHANG Zhimin;JIANG Yan;DI Yu;XU Qingqing;PAN Pinliang;QIU Maofeng(National AIDS Reference Laboratory,National Center for AIDS/STD Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Fengtai District Center for Disease Control and Prevention,Beijing 100071)

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心参比实验室,北京102206 [2]北京市丰台区疾病预防控制中心微生物检验科,北京100071

出　　处：《中国艾滋病性病》2020年第4期337-340,共4页Chinese Journal of Aids & STD

基　　金：国家“十三五”科技重大专项(2017ZX10201101-002-003)。

摘　　要：目的分析我国部分地区采供血机构发现1型艾滋病病毒(HIV-1)感染者的毒株基因亚型。方法收集2011-2015年重庆等14个省(自治区、直辖市)采供血机构发现的部分HIV-1感染者血浆样品,提取总核糖核酸(RNA),反转录后经巢式聚合酶链反应(PCR)扩增HIV-1 pol基因区片段。PCR产物测序后进行序列分析,计算pol基因区样本间基因离散率,并做系统进化树分析。结果共检测220份血浆样品,有173份经PCR扩增成功,其中60.1%(104份)来自西南地区(重庆、云南)、14.5%(25份)来自华东地区(江西、福建)、12.1%(21份)来自华北地区(天津、河北)、8.1%(14份)来自西北地区(青海、甘肃和新疆)、2.3%(4份)来自华南地区(海南)、2.3%(4份)来自东北地区(吉林、辽宁和内蒙古)、0.6%(1份)来自华中地区(湖北)。检出的基因亚型有8种,其中CRF07BC(50.9%,88份)和CRF01AE(28.9%,50份)所占比例较大,随后依次为CRF08BC(11.0%,19份)、CRF5501B(2.9%,5份)、B(2.9%,5份)、CRF85BC(0.6%,1份)、CRF6801B(0.6%,1份)和C(0.6%,1份);另有3份样品的基因亚型为未知亚型。近年在男男性行为者(MSM)中新被检出并流行的有CRF5501B和CRF6801B亚型。结论我国献血人群HIV-1感染者毒株的基因亚型分布日益多样化;主要流行于MSM的两种基因亚型在献血人群中被检出,提示采供血机构应进一步加强对献血员的背景调查和筛选。Objective To analyze HIV-1 subtypes in infected people detected from blood stations in some regions of China. Methods Plasma specimens were collected from HIV-1 infections detected from blood stations in Chongqing and other 13 provinces between 2011 and 2015. The total RNAs were extracted from plasma specimens. The viral pol gene region fragments were amplified by nested polymerase chain reaction(PCR) after reverse transcription, sequenced and analyzed for HIV-1 subtypes. The gene distance between pol gene regions was calculated and phylogenetic tree analysis was performed. Results PCR products and pol gene sequences were successfully obtained from 173 out of 220 specimens, in which 60.1%(104), 14.5%(25), 12.1%(21), 8.1%(14), 2.3%(4), 2.3%(4) and 0.6%(1) were from Southwest, East, North, Northwest, South, Northeast and Central China, respectively. The subtypes CRF07BC, CRF01AE, CRF08BC, CRF5501B, CRF85BC, CRF6801B, B and C were detected, accounting for 50.9%(88), 28.9%(50), 11.0%(19), 2.9%(5), 0.6%(1), 0.6%(1), 2.9%(5) and 0.6%(1), respectively. Subtypes CRF5501B and CRF6801B were newly found and mostly transmitted in men who had sex with man(MSM) in recent years. Conclusion The distribution of HIV-1 subtypes among blood donors in China has become increasingly diversified. Two HIV-1 subtypes mostly transmitted between MSM were found in blood donors, indicating that measures should be further strengthened to know the background of blood donors and the screening should be more rigorous.

关 键 词：1型艾滋病病毒 基因亚型 系统进化树 献血者 

分 类 号：R512.91[医药卫生—内科学] R373.9[医药卫生—临床医学]