膜黄体酮受体β和γ在子宫内膜癌中的定位及对其预后的影响  

Localization of Membrane Progesterone Receptor and in Endometrial Carcinoma and Its Influence on Prognosis

作　　者：陈忠平 赵立霞 张家勇 CHEN Zhongping;ZHAO Lixia;ZHANG Jiayong(He'nan Shengde Hospital,Xinyang,464100)

机构地区：[1]河南圣德医院,464100

出　　处：《实用癌症杂志》2020年第6期871-874,共4页The Practical Journal of Cancer

基　　金：国家自然科学基金青年基金项目(编号:81502373)。

摘　　要：目的探讨膜黄体酮受体(mPR)β和γ在子宫内膜癌(EC)中的定位及对其预后的影响。方法选择经妇产科手术治疗的EC患者60例,从术中获取EC组织样本以及癌旁组织。实时荧光定量PCR检测PAQR7、PAQR8和PAQR5基因的表达,免疫组化检测mPRα、mPRβ和mPRγ的表达及定位。结果PAQR7和PAQR8在EC组织中的表达显著低于其在癌旁组织中的表达(P<0.05),PAQR5在EC组织中的表达与在癌旁组织中的表达无显著差异。PAQR5在FIGO分期ⅠA患者EC组织中的表达显著高于其在其他FIGO分期患者中的表达(P<0.05)。mPRα和mPRβ在EC组织中的表达显著低于其在癌旁组织中的表达(P<0.05),mPRγ在EC组织中的表达显著高于其在癌旁组织中的表达(P<0.05)。mPRβ在淋巴管浸润患者EC组织中的表达显著低于其在未发生淋巴管浸润患者中的表达(P<0.05)。免疫组化结果显示mPRs在EC组织和癌旁组织中的定位多样化。在癌旁组织中,mPRα和mPRβ主要定位于细胞膜,而mPRγ定位于细胞质和/或细胞核中。在EC组织中,在细胞膜和/或细胞质中检测到mPRα和mPRβ,而mPRγ仅位于细胞质中。结论mPRs通过影响癌症的进展参与EC发病机制。mPRβ和mPRγ作为EC预后生物标志物的潜在作用还需要进一步大量样品评估。Objective To investigate the localization of membrane progesterone receptor and in endometrial carcinoma(EC)and its influence on prognosis.Methods 60 patients with EC who underwent gynecological surgery were enrolled,and EC tissue samples and adjacent tissues were obtained from surgery.Real-time quantitative PCR was used to detect the expressions of PAQR7,PAQR8 and PAQR5 genes,and the expression and localization of mPR,mPR and mPR were detected by immunohistochemistry.Results The expressions of PAQR7 and PAQR8 in EC tissues were significantly lower than that in adjacent tissues(P<0.05).The expression of PAQR5 in EC tissues was not significantly different from that in adjacent tissues.The expression of PAQR5 in EC tissues of patients with FIGO stageⅠA was significantly higher than that of other FIGO stage patients(P<0.05).The expressions of mPR and mPR in EC tissues were significantly lower than that in adjacent tissues(P<0.05),and the expression of mPR in EC tissues was significantly higher than that in adjacent tissues(P<0.05).The expression of mPR in EC tissues of patients with lymphovascular invasion was significantly lower than that in patients without lymphovascular invasion(P<0.05).The results with immunohistochemistry revealed a diverse localization of mPRs in EC and paracancerous tissues.In adjacent tissues,mPR and mPR are mainly localized in the cell membrane,while mPR is localized in the cytoplasm and/or nucleus.In EC tissues,mPR and mPR are detected in the cell membrane and/or cytoplasm,while mPR is only located in the cytoplasm.Conclusion mPRs participate in the pathogenesis of EC by affecting the progression of cancer.The potential role of mPR and mPR as a biomarker for EC prognosis requires further extensive sample evaluation.

关 键 词：膜黄体酮受体 子宫内膜癌 定位 预后 

分 类 号：R737.33[医药卫生—肿瘤]