石家庄地区已知HLA型别单采血小板捐献者基因资料库库容评价  

Evaluation of A Known HLA Typing Apheresis Platelet Donors Gene Database Pool Size in Shijiazhuang

作　　者：苏蔓[1] 李茵[1] 赵倩[1] 胡光磊[1] 王振雷[1] 何路军[1] SU Man;LI Yin;ZHAO Qian(Organ Transplantation Matching Laboratory,Hebei Blood Center,Shijiazhuang 050071)

机构地区：[1]河北省血液中心,石家庄050071

出　　处：《临床输血与检验》2020年第3期290-294,共5页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：2018年度河北医学适用跟踪项目(No.G2018088)资助。

摘　　要：目的 分析石家庄地区单采血小板捐献者HLA-I类基因(A、B位点)分布特点,探讨在该地区血小板捐献者基因资料库中找到HLA-I类基因(A、B位点)相合供者的概率并进行血小板捐献者基因资料库最小规模评价。方法 对石家庄地区1 935位无血缘关系的血小板捐献者HLA-I 类基因A、B位点基因分型采用聚合酶链反应-序列特异引物(PCR-SSP)和聚合酶链反应-直接测序分型(PCR-SBT)方法,得到基因型后转化为对应的抗原特异性,计算基因频率和单体型频率,进行血小板捐献者基因资料库最小规模评价,并统计交叉反应组(CREG)表型频率及不配合率。结果 1 935位无血缘关系的血小板捐献者中共检出HLA-A位点抗原特异性19种,其中频率最高的抗原为A2(0.313 4)和A11(0.158 4);检出HLA-B 位点抗原特异性43种,其中频率最高的为B13(0.118 1)和B61(0.086 3);观察到HLA-A-B单体型355种,频率大于0.01的共28种,其中频率最高的为A2-B13(0.044 4)和A2-B62(0.029 5);石家庄地区单采血小板捐献者A-A、A-B抗原CREG表位不配合的概率最高分别为1C-2C和7C-5C,Broad CREG不配合概率最高的为4C-6C。结论 理论上石家庄地区建立一个总库容1 500人的血小板捐献者基因资料库有93.18%的患者有95%的可能性找到至少1例HLA相合的供者,本研究1 935人的基因资料库基本可以满足本地区血小板输注无效患者寻找HLA相合供者的需求。Objective To analyze the distribution characteristics of HLA-I loci(A,B)in apheresis platelet donors in Shijiazhuan area,explore the probability of finding an HLA-matched platelet donor in platelet donor pools in Shijiazhuang area,and evaluate the minimum pool size of platelet donor gene database.Methods HLA-I loci A and B genotypes of 1935 unrelated platelet donors in Shijiazhuang area were performed by polymerase chain reaction(PCR)using sequence-specific primers(PCR-SSP)and sequence-based typing(PCR-SBT)methods.HLA genotype obtained were converted to antigenic specificity,calculated genetype frequency and haplotype frequency,and evaluated the minimum pool size of platelet donor gene database.The cross-reactive group(CREG)phenotype frequency and its mismatch rate were counted.Results A total of 19 HLA-A locus phenotype specificities were identified in 1935 unrelated platelet donors,and the highest frequency antigens are A2(0.3134)and A11(0.1584).A total of 43 B-locus phenotype specificities were identified,and the highest frequency antigens are B13(0.1181)and B61(0.0863).355 HLA-A-B haplotypes were observed,of which 28 frequencies were greater than 0.01,the highest frequencies haplotypes were A2-B13(0.0444)and A2-B62(0.0295).1C-2C and 7C-5C have the highest mismatch rate of CREG epitopes of A-A and A-B antigens,and 4C-6C has the highest mismatch rate of Broad CREG in apheresis platelet donors in Shijiazhuang area.Conclusion In theory,93.18%of patients have a 95%;probability to find at least one HLA-matched donor in a platelet donor pool with a total storage capacity of 1,500 patients.The platelet donor pool size of 1935 would basically meet the needs of patients with platelet transfusion refractoriness in Shijiazhuang area.

关 键 词：血小板 HLA-I类基因 基因频率 单体型 CREG 

分 类 号：R692.5[医药卫生—泌尿科学]