Hsa_circRNA_102610 upregulation in Crohn’s disease promotes transforming growth factor-β1-induced epithelial-mesenchymal transition via sponging of hsa-miR-130a-3p  

作　　者：Juan Yin Yu-Lan Ye Tong Hu Li-Juan Xu Li-Ping Zhang Ru-Ning Ji Ping Li Qian Chen Jian-Yun Zhu Zhi Pang 

机构地区：[1]Department of Digestive Disease and Nutrition Research Center,the Affiliated Suzhou Hospital of Nanjing Medical University,Suzhou 215008,Jiangsu Province,China [2]Department of Gastroenterology,the Affiliated Suzhou Hospital of Nanjing Medical University,Suzhou 215008,Jiangsu Province,China [3]Department of Biomedical Engineering,the Affiliated Suzhou Hospital of Nanjing Medical University,Suzhou 215008,Jiangsu Province,China

出　　处：《World Journal of Gastroenterology》2020年第22期3034-3055,共22页世界胃肠病学杂志（英文版）

基　　金：Supported by the Suzhou Special Project of Diagnosis and Treatment for Key Clinical Disease,No.LCZX201715;the Natural Science Foundation of Jiangsu Province,No.BK20161232;the Science and Technology Development Fund of Nanjing Medical University,No.NMUB2018215.

摘　　要：BACKGROUND The incidence of inflammatory bowel disease,a chronic intestinal inflammatory disorder that includes Crohn’s disease(CD)and ulcerative colitis,is rising.Circular RNAs are considered valuable diagnostic biomarkers for CD.Current evidence supports the views that epithelial-mesenchymal transition(EMT)plays an important role in CD pathogenesis,and that hsa-miR-130a-3p can inhibit transforming growth factor-β1(TGF-β1)-induced EMT.Our previous study revealed that hsa_circRNA_102610 was upregulated in CD patients.Moreover,we predicted an interaction between hsa_circRNA_102610 and hsa-miR-130a-3p.Thus,we hypothesized that hsa_circRNA_102610 may play roles in the proliferation and EMT of intestinal epithelial cells by sponging hsa-miR-130a-3p to participate in the pathogenesis of CD.AIM To explore the mechanism of hsa_circRNA_102610 in the pathogenesis of CD.METHODS The relative expression levels of hsa_circRNA_102610 and hsa-miR-130a-3p in patients were detected by quantitative reverse transcription-polymerase chain reaction.The proliferation of human intestinal epithelial cells(HIECs)and normal-derived colon mucosa cell line 460(NCM460)cells was detected by cell counting kit-8,5-ethynyl-2’-deoxyuridine staining and cell cycle assays following overexpression or downregulation of hsa_circRNA_102610.Cell proliferation assays were performed as described above in a rescue experiment with hsa-miR-130a-3p mimics.The interaction of hsa_circRNA_102610 and hsa-miR-130a-3p was verified by fluorescence in situ hybridization and dual luciferase reporter assays.The relative expression levels of CyclinD1,mothers against decapentaplegic homolog 4(SMAD4),E-cadherin,N-cadherin and Vimentin were detected by western blotting following hsa_circRNA_102610 overexpression,TGF-β1-induced EMT or hsa-miR-130a-3p mimic transfection(in rescue experiments).RESULTS Upregulation of hsa_circRNA_102610 was determined to be positively correlated with elevated fecal calprotectin levels in CD(r=0.359,P=0.007)by Pearson correlation analysi

关 键 词：Hsa_circRNA_102610 Hsa-miR-130a-3p Epithelial-mesenchymal transition Crohn’s disease Mothers against decapentaplegic homolog 4 Transforming growth factor-β1 

分 类 号：R574.62[医药卫生—消化系统]