Cross electro-nape-acupuncture ameliorates cerebral hemorrhageinduced brain damage by inhibiting necroptosis  

作　　者：Guo-Feng Cai Zhong-Ren Sun Zhe Zhuang Hai-Chun Zhou Shan Gao Kai Liu Li-Li Shang Kun-Ping Jia Xiu-Zhen Wang Hui Zhao Guo-Liang Cai Wen-Li Song Sheng-Nan Xu 

机构地区：[1]Hanan Branch of Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150001,Heilongjiang Province,China [2]Postdoctoral Research Station of Heilongjiang Academy of Traditional Chinese Medicine,Harbin 150001,Heilongjiang Province,China [3]Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,Heilongjiang Province,China [4]Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150000,Heilongjiang Province,China [5]First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,Heilongjiang Province,China [6]Harbin Sport University,Harbin 150001,Heilongjiang Province,China [7]Graduate School of Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,Heilongjiang Province,China

出　　处：《World Journal of Clinical Cases》2020年第10期1848-1858,共11页世界临床病例杂志

基　　金：Supported by State Administration of Traditional Chinese Medicine of Heilongjiang Province,No.ZHY16-027;Harbin Municipal Science and Technology BureauYouth Reserve Talent Project,No.2017RAQXJ170

摘　　要：BACKGROUND Receptor interacting protein kinase 1(RIPK1)-mediated cell death,including apoptosis and necroptosis,belongs to programmed cell death.It has been reported that RIPK1-mediated necroptosis exists in lesions of cerebral hemorrhage(CH).Electroacupuncture,a treatment derived from traditional Chinese medicine,could improve neurological impairment in patients with brain injury.AIM To investigate the protective role of cross electro-nape acupuncture(CENA)in CH,and clarify the potential mechanism.METHODS CH rat models were established,and CENA was applied to the experimental rats.Neurological functions and encephaledema were then measured.Necrotic cells in the brain of rats with CH were evaluated by propidium iodide staining.Necroptosis was assessed by immunofluorescence.Activation of the necroptosisrelated pathway was detected by western blot.Extraction of brain tissue,cerebrospinal fluid and serum samples was conducted to measure the expression and secretion of inflammatory cytokines by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay.RESULTS The necroptotic marker p-MLKL was detectable in the brains of rats with CH.Next,we found that CENA could ameliorate neurological functions in rat models of CH.Moreover,the upregulation of RIPK1-mediated necroptosis-related molecules in the brains of rats with CH were inhibited by CENA.Further investigation revealed that CENA partially blocked the interaction between RIPK1 and RIPK3.Finally,in vivo assays showed that CENA decreased the expression of the inflammatory cytokines tumor necrosis factor-α,interleukin-6 and interleukin-8 in CH rat models.CONCLUSION These findings revealed that CENA exerts a protective role in CH models by inhibiting RIPK1-mediated necroptosis.

关 键 词：Cross electro-nape acupuncture Cerebral hemorrhage Receptor interacting protein kinase 1 NECROPTOSIS Quantitative real-time polymerase chain reaction Enzymelinked immunosorbent assay 

分 类 号：R245[医药卫生—针灸推拿学]