细胞外基质蛋白DMP1-C和DMP1-N在咬合创伤治疗前后大鼠髁突软骨内的差异表达  

Differential Expression of Extracellular Matrix Protein DMP1-C and DMP1-N in Condylar Cartilage Before and After Occlusal Trauma Treatment

作　　者：刘培红[1] 马肃[1] 李永恒 王海侠[3] LIU Peihong;MA Su;LI Yongheng;WANG Haixia(Department of Periodontology, College of Stomatology, Harbin Medical University, Harbin 150001, China;Department of Stomatology, Qunli Hospital, First Affiliated Hospital of Harbin Medical University, Harbin 150070, China;Heilongjiang Scholars Laboratory, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China)

机构地区：[1]哈尔滨医科大学附属第一医院牙周病科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第一医院群力分院口腔科,黑龙江哈尔滨150070 [3]哈尔滨医科大学附属第一医院龙江学者实验室,黑龙江哈尔滨150001

出　　处：《口腔医学研究》2020年第5期443-448,共6页Journal of Oral Science Research

基　　金：黑龙江省自然科学基金面上项目(编号:H2017030);黑龙江省教育厅面上项目(编号:12531401)。

摘　　要：目的:通过观察咬合创伤治疗前后大鼠髁突软骨和软骨下骨小梁组织学变化以及细胞外基质蛋白DMP1-C和DMP1-N在髁突软骨内的差异表达,分析咬合创伤对大鼠髁突矿化影响的分子机制,并明确调牙合治疗的临床意义。方法:建立咬合创伤2周、咬合创伤2周去除创伤恢复2周和恢复4周大鼠模型,H&E染色观察髁突前、中、后部软骨及软骨下骨小梁组织学变化,免疫组化染色观察细胞外基质蛋白DMP1-C和DMP1-N在咬合创伤治疗前后髁突软骨前、中、后部的差异表达。结果:与对照组大鼠相比较,咬合创伤2周组大鼠髁突变化以髁突前部最为显著:髁突软骨肥大细胞层变薄或消失,钙化软骨层明显增厚,髁突软骨细胞排列紊乱;DMP1-C蛋白表达增加、DMP1-N蛋白表达显著减少;软骨下骨小梁排列不规则。治疗2周组大鼠髁突软骨肥大细胞层和钙化软骨层厚度逐渐恢复,髁突软骨细胞排列趋于规则、多沿受力方向分布;DMP1-C蛋白表达比创伤2周组减少、仍多于对照组,DMP1-N蛋白表达多于对照组。治疗4周组大鼠髁突软骨厚度、细胞层数、软骨细胞分布以及DMP1-C和DMP1-N蛋白的表达与对照组大鼠无显著差异。结论:咬合创伤使大鼠髁突软骨内成骨进程加快,伴随着细胞外基质矿化蛋白DMP1-C和DMP1-N表达变化,模拟调牙合治疗后,髁突组织形态、软骨下骨矿化和DMP1蛋白的表达可恢复正常。Objective:To analyze the molecular mechanism of occlusal trauma on condylar mineralization in rats and to determine the clinical significance of occlusal adjustment through observing the histological changes of rats condylar cartilage and subchondral trabeculae and the differential expression of ECM protein DMP1-C and DMP1-N terminal in condylar cartilage.Methods:The rat models with 2 weeks occlusal trauma,2 weeks after removal of 2 weeks occlusal trauma,and 4 weeks after removal of 2 weeks occlusal trauma.HE staining was used to observe the histological changes of condylar cartilage and subchondral trabeculae among the anterior,middle,and posterior of the condylar process,and immunohistochemistry was used to observe the differential expression of ECM protein DMP1-C and DMP1-N among the anterior,middle,and posterior of the condylar process before and after traumatic treatment.Results:Compared with the control group,the changes of condylar process in 2 weeks occlusal trauma group were significantly prominent in anterior area:mastocyte regions were widen or disappearing,calcified cartilage regions were significantly widen,condylar cartilage cells were in disorder;the expression of DMP1-C increased significantly,and the expression of DMP1-N decreased significantly.Subchondral trabeculae was in no orderly pattern.The rats condylar cartilage mastocyte regions and the calcified cartilage regions in 2 weeks treatment group were gradually recovered,and the arrangement of condylar cartilage cells become regular and gradually distributed along the stress direction;the expression of DMP1-C was lower than 2 weeks occlusal traumatism group but higher than normal control group.There were no significant difference between 4 weeks treatment group and control group in the thickness of condylar cartilage,the layers of cells,the distribution of cartilage cell and the expression of DMP1-C and DMP1-N.Conclusion:Occlusal trauma accelerated the process of endochondral ossification in rats condylar process,with the changes of the expres

关 键 词：咬合创伤 髁突 调牙合 DMP1 软骨内成骨 

分 类 号：R78[医药卫生—口腔医学]