UPLC-MS/MS测定水源水中17种氨基酸及其应用  

Determination of 17 Amino Acids in Source Water by UPLC-MS/MS and Its Application

作　　者：王钰芳 贝尔 邱玉 汪隽 齐飞[1] 陈超 WANG Yu-fang;BEI Er;QIU Yu;WANG Jun;QI Fei;CHEN Chao(College of Environmental Science and Engineering,Beijing Forestry University,Beijing 100083,China;State Key Joint Laboratory of Environmental Simulation and Pollution Control,School of Environment,Tsinghua University,Beijing 100084,China)

机构地区：[1]北京林业大学环境科学与工程学院,北京100083 [2]清华大学环境学院环境模拟与污染控制国家重点联合实验室,北京100084

出　　处：《中国给水排水》2020年第8期106-112,118,共8页China Water & Wastewater

基　　金：国家自然科学基金资助面上项目(21777079)。

摘　　要：氨基酸是水中重要的含氮有机物,也是部分含氮消毒副产物的前体物。为了解国内实际水源水中溶解性氨基酸的存在情况,建立了超高效液相色谱串联质谱(UPLC-MS/MS)直接测定水中17种氨基酸的检测方法,并对不同流域水源水中的溶解态氨基酸存在情况进行了研究。该方法的线性范围为1~200 nmol/L,检出限为0.2~1.5 nmol/L,相关系数(R2)均大于0.99,方法精密度在0.11%~4.66%之间,加标回收率在88.31%~114.92%之间,满足水环境中氨基酸分析的要求,为地表水的氨基酸浓度检测提供了简便、可靠的方案。采用该方法检测实际样品的结果表明,长江、汉江、东江和千岛湖水中溶解性氨基酸总浓度分别为1296、1348、885和55 nmol/L。Amino acids are important part of nitrogen-containing organic matters in water and precursors of nitrogen-containing disinfection by-products.In order to understand the existence of dissolved amino acids in source water of China,a method for determining 17 amino acids in water by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was established,and the occurrence of amino acids in different source waters was studied.The results showed that the linear range of the method was 1-200 nmol/L,the detection limit was 0.2-1.5 nmol/L,the coefficient of correlation(R2)was greater than 0.99,the precision of the method was from 0.11%to 4.66%,and the recovery ratio of the spiking samples were from 88.31%to 114.92%,which could meet the requirements of amino acids detection in water environment.This method provided a simple and reliable plan for the detection of amino acid concentration in surface water.The results of the actual sample showed that the total concentrations of dissolved amino acids in the Yangtze,Hanjiang,Dongjiang and Qiandao Lake waters were 1296 nmol/L,1348 nmol/L,885 nmol/L and 55 nmol/L,respectively.

关 键 词：水源水 氨基酸 超高效液相色谱串联质谱 

分 类 号：TU991[建筑科学—市政工程]