HPLC法测定理气舒心片中阿魏酸的含量  

Simultaneous Determination of Ferulic Acid in LiQi ShuXin Tablet by HPLC

作　　者：袁文洪 高宇明 谢艳 何琴 代仕梅 吴秀清 YUAN Wen-hong;GAO Yu-ming;XIE Yan;HE Qin;DAI Shi-mei;WU Xiu-qing

机构地区：[1]资阳市食品药品检验检测中心,四川资阳641399

出　　处：《海峡药学》2020年第5期54-55,共2页Strait Pharmaceutical Journal

摘　　要：目的采用HPLC法测定理气舒心片中阿魏酸的含量。方法该药物70%甲醇提取液分析采样色谱柱Waters XSelect HSS T3(4.6×250mm,5μm);流动相:甲醇-水(25∶75)为流动相,等度洗脱;流速:1.0mL·min^-1;检测波长:316nm;柱温:30℃。结果阿魏酸对照品在0.449~22.473μg·mL^-1浓度范围内与峰面积线性关系良好(r=1);平均回收率为101.77%(RSD=1.59%)。结论所采用的方法准确、可靠,可行性及重复性良好,能有效控制理气舒心片的质量。OBJECTIVE To establish a HPLC method for simultaneous content determination of ferulic acid in LiQi ShuXin tablet.METHODS The analysis of methanol-water(25∶75)extract of LiQi ShuXin tablet was performed on a 30℃thermostatic InertsiL Waters XSelect HSS T3(4.6×250mm,5μm),with the mobile phase comprising of methanol-water flowing at 1.0mL·min^-1.RESULTS LiQi ShuXin tablet showed good linear relationships within the range of 0.449-22.473μg·mL^-1(r=1),whose average recoveries were 101.77%,with the RSD of 1.59%.CONCLUSION This sensitive,teliable and accurate mehtod can be used for the quality control of LiQi ShuXin tablet.

关 键 词：理气舒心片 阿魏酸 高效液相色谱法 

分 类 号：R927[医药卫生—药学]