甲基硒酸结合低剂量照射对口腔癌细胞增殖、凋亡的影响  

Effects of methylselenoic acid combined with low-dose irradiation on proliferation and apoptosis of oral cancer cells

作　　者：董阳阳 朱晓斌 杨志诚 DONG Yang-yang;ZHU Xiao-bin;YANG Zhi-cheng(Department of Stomatology,Binzhou Medical University,Binzhou 256603,Shandong,China;Department of Stomatology,Fengcheng Health Center of Jimo District,Qingdao 266200,Shandong,China;Department of Stomatology,Shanghai Dental Hospital,Shanghai 200001,China)

机构地区：[1]滨州医学院口腔医学院,山东滨州256603 [2]即墨区丰城卫生院口腔科,山东青岛266200 [3]上海市口腔医院口腔科,上海200001

出　　处：《现代医学与健康研究电子杂志》2020年第1期5-7,共3页Modern Medicine and Health Research

摘　　要：目的探讨MSA结合低剂量照射对口腔癌细胞增殖、凋亡的影响。方法体外培养口腔癌KB细胞,实验分为MSA-Radiation组和MSA组,分别给予0μmol/L、0.2μmol/L、0.4μmol/L、0.8μmol/L、1.2μmol/L的MSA干预,MSA-Radiation组同时行0.08 Gy的低剂量照射。MTT法检测各组增殖抑制率,Annexin V-FITC/PI检测细胞AR。结果与0μmol/L MSA干预相比,0.2μmol/L、0.4μmol/L、0.8μmol/L、1.2μmol/L MSA干预的MSA-Radiation组和MSA组的增殖抑制率明显升高,且呈剂量依赖性;MSA-Radiation组的增殖抑制率均高于相同浓度MSA干预的MSA组(P<0.05)。与0μmol/LMSA干预相比,0.2μmol/L、0.4μmol/L、0.8μmol/L、1.2μmol/L MSA干预的MSA-Radiation组和MSA组的AR明显升高,且呈剂量依赖性;MSA-Radiation组的AR均高于相同浓度MSA干预的MSA组(P<0.05)。结论相比单独MSA干预,MSA和低剂量照射结合可以更有效抑制KB细胞的增殖,促进细胞的凋亡,且呈现MSA剂量依赖性。Objective To investigate the effect of methylselenoic acid(MSA) combined with low-dose irradiation on the proliferation and apoptosis of oral cancer cells. Methods KB cells of oral cancer were cultured in vitro, the experiments were divided into MSA-Radiation group and MSA group, which were intervened with 0 μmol/L, 0.2 μmol/L, 0.4 μmol/L, 0.8 μmol/L, 1.2 μmol/L MSA respectively. MSARadiation group received low-dose irradiation with 0.08 Gy at the same time. MTT method was used to detect the proliferation inhibition rate of each group, and Annexin V-FITC/PI was used to detect cell AR. Results Compared with the 0 μmol/L MSA, the proliferation inhibition rates of the MSA-Radiation group and the MSA group significantly increased in a dose-dependent manner;the proliferation inhibition rates of the MSA-Radiation group were higher than those of the MSA group treated with the same concentration(P<0.05). Compared with the 0 μmol/L MSA intervention, the AR in the MSA-Radiation group and the MSA group significantly increased in a dose-dependent manner;the AR in the MSA-Radiation group was higher than that in the MSA group treated with the same concentration of MSA(P<0.05). Conclusion Compared with MSA intervention alone, the combination of MSA and low-dose irradiation can more effectively inhibit the proliferation of KB cells, promote cell apoptosis, and present a MSA dose-dependent manner.

关 键 词：甲基硒酸 低剂量照射 口腔癌细胞 增殖 凋亡 

分 类 号：R73-36[医药卫生—肿瘤]