1株登革Ⅰ型病毒的分离鉴定及系统进化分析  

Isolation,identification and phylogenetic analysis of a dengue virus Ⅰ

作　　者：王宇航 张星艳 花群义 任琴 戴少波 李体远[1] 金宁一 周伯平 吴诗品[1] 李昌 杜寿文 Wang Yuhang;Zhang Xingyan;Hua Qunyi;Ren Qin;Dai Shaobo;Li Tiyuan;Jin Ningyi;Zhou Boping;Wu Shipin;Li Chang;Du Shouwen(Department of Infectious Diseases,the Second Clinical Medical College of Jinan University,Shenzhen People's Hospital,Guangdong Shenzhen 518020,China;Animal and Plant Inspection and Quarantine Technology Center,Shenzhen Customs District P.R.C.,Guangdong Shenzhen 518000,China;Key Laboratory of Jilin Province for Zoonosis Prevention and Control,Institute of Military Veterinary Medicine,Academy of Military Medical Sciences,Changchun 130062,China)

机构地区：[1]暨南大学第二临床医学院、深圳市人民医院感染内科,广东深圳518020 [2]深圳海关动植物检验检疫技术中心,广东深圳518000 [3]军事科学院军事医学研究院军事兽医研究所、省部共建吉林省人兽共患病预防与控制重点实验室,长春130062

出　　处：《新发传染病电子杂志》2020年第2期100-103,共4页Electronic Journal of Emerging Infectious Diseases

基　　金：国家自然科学基金(31702210,31972719);深圳市政府卫生“三名工程”专项基金(SZSM201612034)。

摘　　要：目的了解1株登革病毒分离株的来源及进化情况。方法分离获得1株登革病毒,通过对该毒株进行血清型分型鉴定及基因组序列测定,进而通过生物信息学方法对囊膜蛋白E进行系统进化分析。结果分离获得1株DENV血清Ⅰ型基因Ⅰ型毒株;且E基因的同源性分析显示,与Malaysia 0802aTw、Singapore株、浙江17-45株、YN176282株、BNJH17108株的核苷酸同源性均在99%以上;氨基酸水平,与NJX-14株、浙江17-45株、Malaysia 0802aTw株、1208aTw株等多种毒株的E蛋白的同源性均为99.8%,表明这些毒株可能源于同一株原始毒株,且E蛋白序列遗传进化稳定,与2002年以来多株广东省分离的毒株高度同源。结论近十年发现在我国的DENVⅠ型基因Ⅰ型遗传进化稳定,保守程度高,有利于针对该血清型登革病毒的防控。Objective To preliminarily understand the origin and evolution of the dengue virus isolated strain.Methods A dengue virus was isolated,and its serotyping and genome sequence was identified and analyzed.Especially,the phylogenetic analysis of envelope protein E was performed by bioinformatics methods,Results A serotypeⅠof DENV isolated strain named as SZ905 was identified to be genotype Ⅰ.And the homology analysis of E gene showed that the nucleotide homology with Malaysia 0802atw strain,Singapore strain,Zhejiang 17-45 strain,YN176282 strain and BNJH 17108 strain were all above 99%,and the homology of E protein with the multiple strains such as NJX-14,Zhejiang 17-45,Malaysia 0802atw and 1208atw strains was all above 99.8%in amino acid level,indicating that these strains maybe originate from a same original strain.And the evolution of E protein sequence is stable and highly homologous with that of several strains isolated in Guangdong Province since 2002.Conclusion DENV-ⅠgenotypeⅠin China has stable genetic evolution and high degree of conservation,which is conducive to the prevention and control of this serotype virus disease in recent years.

关 键 词：登革病毒 基因型 系统进化 

分 类 号：R446.5[医药卫生—诊断学] R512.8[医药卫生—临床医学]