硫醇修饰Fe3O4@SiO2@Ag SERS基底检测氨苄西林  

Thiol-modified Fe3O4@SiO2@Ag for SERS detection of ampicillin

作　　者：卢浩 孔红星[1,2] 李利军 冯军[1,2] 程昊 徐娅娟[1,2] LU Hao;KONG Hongxing;LI Lijun;FENG Jun;CHENG Hao;XU Yajuan(Guangxi Key Laboratory of Green Processing of Sugar Resources,College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006;Province and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry,Nanning 530004)

机构地区：[1]广西糖资源绿色加工重点实验室,广西科技大学生物与化学工程学院,柳州545006 [2]蔗糖产业省部共建协同创新中心,南宁530004

出　　处：《分析试验室》2020年第5期541-545,共5页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金地区科学基金(31560466);广西高等学校高水平创新团队及卓越学者计划项目(桂教人[2014]7号)资助。

摘　　要：制备了一种烷基硫醇修饰的磁性纳米银SERS基底,对氨苄西林(AMP)进行了拉曼检测,并考察了不同的硫醇修饰磁性纳米银基底对AMP的增强效果。采用不同烷基链长的硫醇对磁性纳米银SERS基底进行修饰,通过分子的自组装,使烷基硫醇自发的生长在磁性纳米银的银壳表面,通过疏水作用,使AM P分子富集于基底的表面,以达到AM P分子拉曼信号增强的检测目的。实验结果表明,3种不同烷基链长的硫醇修饰的基底对AMP的拉曼增强效果依次是正丙硫醇>正己硫醇>正壬硫醇。正丙硫醇修饰的基底对AMP盐酸溶液的检出限为0. 1 nmol/L。通过硫醇修饰的磁性纳米银基底能够富集AMP分子,可用于低浓度检测。An alkylthiol-modified magnetic nano-silver SERS substrate was fabricated for Raman detection of ampicillin( AMP),and the enhancement effect of different thiol-modified magnetic nano-silver substrates on AMP were compared. The magnetic nano-silver SERS substrate was modified by different alkyl chain length thiols. The self-assembly of the molecules allowed the alkyl thiol to spontaneously grow on the silver shell surface of the magnetic nano-silver,and the AMP molecule was enriched by hydrophobic interaction on the surface of the substrate to achieve the detection of Raman signal enhancement of AMP molecules. The experimental results showed that the Raman-enhancing effect of thiol-modified substrates with three different alkyl chain lengths on AMP was 1-Propanethiol > 1-hexanethiol > 1-nonanethiol. The detection limit of the positive propyl thiol modified substrate to the AMP hydrochloric acid solution was 0. 1 nmol/L. The results showed that the thiol-modified magnetic nano-silver substrate can enrich AMP molecules and can be used for low concentration detection.

关 键 词：表面增强拉曼散射 氨苄西林 烷基硫醇 

分 类 号：O657.63[理学—分析化学]