香露兜粉对大鼠致畸性研究  

Study on teratogenicity of Pandanus amaryllifolius Roxb. powder in rats

作　　者：冯丁山[1] 黄业宇[1] 苏林梁[1] 吴爱琴[1] 张晓昕[1] 王湛[1] 林卫华[1] FENG Dingshan;HUANG Yeyu;SU Linliang;WU Aiqin;ZHANG Xiaoxin;WANG Zhan;LIN Weihua(Hainan Provincial Centre for Disease Control and Prevention,Hainan Haikou 570203,China)

机构地区：[1]海南省疾病预防控制中心,海南海口570203

出　　处：《中国食品卫生杂志》2020年第2期130-133,共4页Chinese Journal of Food Hygiene

摘　　要：目的评价食用香露兜粉对人体的母体毒性、胚胎毒性和致畸性。方法采用GB 15193.14-2015《食品安全国家标准致畸试验》中的方法,将已受孕的SD大鼠随机分为溶媒对照组、阳性对照组以及香露兜粉样品高剂量组、中剂量组、低剂量组(分别为2.50、1.25和0.60 g/kg BW),每组16只。于受孕第6天~第15天,采用灌胃方式经口给予受试物,溶媒对照组用等量纯水,阳性对照组腹腔注射环磷酰胺15 mg/kg。受孕第20天处死孕鼠,检查、解剖并记录胎鼠的畸形情况。结果香露兜粉各剂量组均未见母体毒性、胚胎毒性和致畸作用,各项指标与溶媒对照组比较,均未发现明显异常,整体分析不具有剂量-反应关系趋势。阳性对照组大鼠有明显的胚胎毒性和致畸毒性。致畸作用的未观察到有害作用剂量(NOAEL)为2.50 g/kg BW。结论在本研究条件下,未见香露兜粉样品具有母体毒性、胚胎毒性和致畸作用。Objective To evaluate whether the Pandanus amaryllifolius Roxb. powder had reproductive toxicity to the SD rat and whether it was teratogenic to theembryo, perform toxicological evaluation of its safety, and provide the basis of the toxicology safety for the development of food additive. Methods Pregnant rats were randomly divided into negative control group, positive control group, Pandanus amaryllifolius Roxb. powder sample high-dose group, middle dose group and low-dose group(2.50, 1.25 and 0.60 g/kg BW respectively),using the teratogenic test method in GB 15193.14-2015. The test substance was orally administered on the 6 th to 15 th day of conception, and was administered by intragastric administration. The vehicle control group was intragastrically administered with the same amount of pure water;the positive control group was intraperitoneally injected with cyclophosphamide 15 mg/kg. The pregnant rats were sacrificed on the 20 th day of pregnancy, and the abnormalities of the fetal rats were examined, dissected and recorded. Results The groups intervened with Pandanus amaryllifolius Roxb. powder showed no producematernal toxicity,mbryonic toxicity nor teratogenicity,compared with the negative control group. No significant abnormality was found in the indicators, and the overall analysis did not have a dose-response relationship. The positive control group showed obvious embryonic toxicity and teratogenicity. The no observed adverse effect level(NOAEL) was 2.50 g/kg BW. Conclusion In the dose range of this experimental study, there was no teratogenic effect in the sample of Pandanus amaryllifolius Roxb. powder.

关 键 词：香露兜粉 大鼠 母体毒性 胚胎毒性 致畸性 

分 类 号：R155[医药卫生—营养与食品卫生学]