莴苣种质资源耐热鉴定评价  

Identification and Evaluation of Heat Tolerance of Lettuce Germplasm Resources

作　　者：火国涛 葛国军[1] 张兆辉[2] 杨华[1] 刘鸿艳[1] 罗利军[1] 魏仕伟[1] 龙萍[1] HUO Guo-tao;GE Guo-jun;ZHANG Zhao-hui;YANG Hua;LIU Hong-yan;LUO Li-jun;WEI Shi-wei;LONG Ping(Shanghai Agrobiological Gene Center,Shanghai 201106;Zhuanghang Integrated Experimental Station,Shanghai Academy of Agricultural Sciences,Shanghai 201415)

机构地区：[1]上海市农业生物基因中心,上海201106 [2]上海市农业科学院庄行综合试验站,上海201415

出　　处：《植物遗传资源学报》2020年第3期637-647,共11页Journal of Plant Genetic Resources

基　　金：上海市科技支撑项目(19391900600);上海市农作物种质资源共享服务平台(18DZ2293700);国家农作物种质资源平台(上海)(NICGR2019-021)。

摘　　要：通过在夏季大棚中连续两年的鉴定与评价,对从美国农业部、世界蔬菜中心和上海市农业生物基因中心保存的253份莴苣材料的28个性状进行多样性分析。结果表明叶姿态、叶裂刻、叶缘波状度、叶泡程度、叶片颜色、叶片形状等性状遗传多样性高。28个性状的变异系数在0.0315~2.7366之间,平均值为0.7444,其中变异系数最小的性状为叶片光泽(0.0315),叶片花青苷显色强度的变异系数最大(2.7366)。28个性状的多样性指数在0.0258~1.9700之间,平均值为0.7663,地上部鲜重的多样性指数最高(1.9700),叶片光泽度的多样性指数最低(0.0258)。通过聚类分析将253份材料分为6大类群,类群Ⅰ含99份材料,类群Ⅱ含38份材料,类群Ⅲ含84份材料,类群Ⅳ含5份材料,类群Ⅴ含9份材料,类群Ⅵ含18份材料。本研究通过对253份材料在夏季高温下进行田间综合鉴定,结合耐热性和抽薹时间的调查,初步筛选出14份耐热性较强、抽薹较晚的优良材料。本研究为耐热莴苣种质创新提供了多样性丰富的研究材料,同时为今后耐热相关基因的克隆与耐热机理的解析奠定了基础。Through two consecutive years evaluation of lettuce grown in summer greenhouse,the diversity of 28 traits of 253 lettuce materials introduced from United States Department of Agriculture,World Vegetable Center,and Shanghai Agrobiological Gene Center was analyzed.The result showed that leaf attitude,leaf lobes,undulation of leaf margin,showiness of leaf pustules,leaf color,leaf shape and some other traits had highly genetic diversity.The coefficient of variation of the 28 character ranged from 0.0315(glossiness of leaf)to 2.7366(intensity of leaf anthocyanin coloration),with the mean value of 0.7444.The diversity index of 28 characters ranged from 0.0258(glossiness of leaf)to 1.9700(fresh weight of aerial parts).According to the cluster analysis,the 253 materials were divided into 6 groups,with group I containing 99 materials,group II 38 materials,group III 84 materials,group IV 5 materials,group V 9 materials,and group VI 18 materials.In this study,the 253 materials were synthetically evaluated in the field under high temperature in summer and 14 excellent materials with strong heat tolerance and late bolting were preliminarily screened based on the investigation of heat damage and bolting time.The identification and evaluation of heat tolerance of lettuce germplasm resources provide variety materials for resource innovation,gene cloning,and mechanism research for lettuce heat tolerance in the future.

关 键 词：高温 莴苣 遗传多样性 耐热 

分 类 号：S636.2[农业科学—蔬菜学]