川黄连对大鼠化学灼烧型口腔溃疡的抗炎作用观察  

Anti-inflammatory Effect of Chuanhuanglian on Chemically Burned Oral Ulcer in Rats

作　　者：李简 苏雪莲[1] 杨华飞 穆晓琴 LI Jian;SU Xue-lian;YANG Hua-fei;MU Xiao-qin(Major in Stomatology,School of Medicine,Northwest University for Nationalities,Lanzhou730010,Gansu,China)

机构地区：[1]西北民族大学医学院口腔医学专业,甘肃兰州730010

出　　处：《医学信息》2020年第11期61-63,共3页Journal of Medical Information

基　　金：国家级大学生创新创业训练计划项目(编号:201910742120);西北民族大学本科生科研创新项目(编号:XBMU-BYL 19171)。

摘　　要：目的探究川黄连对大鼠化学灼烧型口腔溃疡的抗炎作用机理。方法利用化学灼烧的方法,在30只SD大鼠的舌背部制造口腔溃疡模型,按照随机抽样分为实验组和对照组,每组15只。实验组使用川黄连粉末直接覆盖于溃疡表面,对照组不进行处理,造模后48 h、第3天,比较两组大鼠水肿面积变化率、口腔溃疡组织学分级,分别以肉眼和切片比较两组大鼠由于化学烧灼而导致的粘膜水肿恢复情况。结果①造模后48 h可见大鼠舌腹粘膜发生炎性水肿反应,水肿部位粘膜泛白,表面多个小水泡,凹凸不平,稍隆起,内含清亮液体,边缘清晰,也可由多个小水泡融合而形成的边缘不规则的较大水泡;造模后第3天,实验组粘膜水肿基本消退,个别水肿未完全消失但也基本局限与溃疡附近;对照组粘膜水肿也有消退,但水肿面积与实验组有所差别。②造模后48 h粘膜水肿形成面积达到最大值,随后逐渐缩小;造模后第3天,实验组水肿面积变化率为(0.873±0.020)%,高于对照组的(0.714±0.064)%,差异有统计学意义(P<0.05)。③实验组口腔溃疡组织学分级中Ⅰ、Ⅱ及大鼠少于对照组,Ⅲ、Ⅳ级多于对照组,差异有统计学意义(P<0.05);HE染色显示,实验组炎细胞浸润程度较低,细胞形态多完整,组织水肿程度低,坏死组织少,部分切片可见肉芽组织充填于溃疡创面;对照组炎细胞浸润程度高,可见部分炎细胞破裂死亡,坏死组织少,鲜见肉芽组织长入溃疡创面。结论川黄连对化学灼烧型口腔溃疡引起的炎症性水肿有促进消退的作用,同时可以降低溃疡组织内炎细胞的浸润。对化学刺激性溃疡有抗感染,抗炎症的作用。Objective To explore the anti-inflammatory mechanism of Chuanhuanglian on chemically burned oral ulcer in rats.Methods Using chemical burning method,an oral ulcer model was created on the back of the tongue of 30 SD rats,which were divided into an experimental group and a control group according to random sampling,with 15 rats in each group.The experimental group used Chuanhuanglian powder to directly cover the surface of the ulcer,and the control group did not process it.At 48 h and 3 d after the model,the rate of change in edema area and the histological grade of oral ulcer were compared between the two groups.Recovery of mucosal edema caused by chemical burning in two groups of rats.Results①At 48 h after modeling,an inflammatory edema reaction occurred in the mucous membrane of the tongue and abdomen of the rat.The mucosa of the edema site was whitened,with multiple small blisters on the surface,uneven,slightly raised,containing clear liquid,clear edges,and can also be fused by multiple small blisters the large blisters with irregular edges formed;on the third day after modeling,the mucosal edema in the experimental group basically subsided,and some individual edema did not completely disappear but was basically limited to the vicinity of the ulcer;the mucosal edema in the control group also subsided,but the edema area was different from the experimental group.②The area of formation of mucosal edema reached the maximum at 48 h after modeling,and then gradually reduced;on the third day after modeling,the rate of change of edema area in the experimental group was(0.873±0.020)%,higher than that of the control group(0.714±0.064)%,the difference was statistically significant(P<0.05).③The histological grade of oral ulcers in the experimental group was less than that of the control groupⅠ,Ⅱand rats,and the gradesⅢandⅣwere more than the control group,the difference was statistically significant(P<0.05);HE staining showed that the degree of inflammatory cell infiltration in the experimental group was lo

关 键 词：口腔溃疡 黄连 化学灼烧 大鼠 

分 类 号：R781.5[医药卫生—口腔医学]