凯里市大气降尘中可培养微生物群落结构分析研究  

Exploring the Community Structure of Culturable Microbial in Atmospheric Deposition in Kaili City

作　　者：李向阳 王楠[1] 朱小章 杨蒙 LI Xiang⁃yang;WANG Nan;ZHU Xiao⁃zhang;YANG Meng(Kaili University,Kaili,Guizhou,556011,China;Fudan University,Yangpu,Shanghai,200433,China)

机构地区：[1]凯里学院大健康学院,贵州凯里556011 [2]复旦大学环境科学与工程系,上海杨浦200433

出　　处：《凯里学院学报》2020年第3期84-90,共7页Journal of Kaili University

基　　金：国家自然科学基金(21507012,21966015);中国博士后科学基金(2015M570329);贵州省科学技术基金计划项目(黔科合基础[2019]1287号);贵州省科技厅联合基金(黔科合LH字[2017]7176);凯里学院博士专项基金(BS201412);凯里学院校级教学改革与研究项目(JG201821)。

摘　　要：为了探究空气质量指数较好区域大气沉降中微生物组成及多样性,以凯里市为代表,通过采集凯里城区、炉山工业区、清水江畔3个典型区域大气降尘颗粒物,采用细菌可培养法、梯度稀释计数法及16S rRNA基因分子鉴定方法对凯里市大气降尘颗粒物中可培养细菌进行群落结构多样性与丰度分析.结果分离得到119株,经分类学鉴定为25属,优势类群为芽孢杆菌、节杆菌及考克氏菌属;城区样品中微生物浓度最高,但是清水江畔样品中微生物种类数量最多.研究结果对认识凯里市大气颗粒物中微生物组分及其多样性具有一定参考价值.To explore the microbial composition and diversity in the atmospheric deposition in the area with better air quality index,in present study,the diversity and abundance of culturable bacterial community in atmospheric deposition sampled from three typical areas of Kaili City(urban areas,Lushan industrial area and Qingshui River side,respectively)were analyzed by the Culture-dependent method,gradient dilution counting method and 16S rRNA gene sequencing method.A total of 119 strains were isolated,and belonged to 25 genera through taxonomic identification.The dominant bacteria were Bacillus,Arthrobacter and Corynebacterium.The urban area sample has the highest concentration of microorganisms,but Qingshui River side sample has the largest number of genera.Taken together,this study provides useful information to better understand the microbial components and diversity in atmospheric in Kaili City.

关 键 词：大气沉降 16S rRNA基因 可培养细菌 凯里市 

分 类 号：X513[环境科学与工程—环境工程] X172