卵巢上皮癌裸鼠原位移植瘤及人卵巢上皮癌卵巢内肿瘤浸润类型分析  

Analysis of neoplasm invasion type in ovary of the orthotopic epithelial ovarian carcinoma model in nude mice and human epithelial ovarian cancer

作　　者：朱根海[1] 王康[1] 符欣蕙[2] 刘富金[3] 陈春英[1] 黄海燕[4] 洪澜[1] 王圣坦[1] 郑朗 Zhu Genhai;Wang Kang;Fu Xinhui;Liu Fujin;Chen Chunying;Huang Haiyan;Hong Lan;Wang Shengtan;Zheng Lang(Department of Gynecology,Hainan General Hospital,Haikou 570311,China;Hainan Institute of Drug Research,Haikou 570311,China;Department of Pathology,Hainan General Hospital,Haikou 570311,China;Research Center,Hainan General Hospital,Haikou 570311,China)

机构地区：[1]海南省人民医院妇科,海口570311 [2]海南省药物研究所,海口570311 [3]海南省人民医院病理科,海口570311 [4]海南省人民医院中心研究室,海口570311

出　　处：《中国医师杂志》2020年第5期699-703,708,共6页Journal of Chinese Physician

基　　金：海南省重点研发项目(ZDYF2017089)。

摘　　要：目的探讨人卵巢上皮癌实体瘤组织片和人卵巢上皮癌细胞株OVCAR-3来源的裸鼠原位移植瘤模型的卵巢内肿瘤浸润类型,以及人卵巢上皮癌卵巢内肿瘤浸润类型的特点。方法将人卵巢上皮癌实体瘤组织片和人卵巢上皮癌细胞株OVCAR-3皮下移植获取瘤源,再分别植入裸鼠单侧卵巢包膜内制备裸鼠原位移植瘤模型。观察两种方法制备的肿瘤模型卵巢内的浸润类型。同时通过病理检查分析了54例国际妇产科联盟(FIGO)Ⅰ~Ⅱ期人卵巢上皮性癌的肿瘤浸润类型。结果肿瘤在卵巢内浸润类型大致分为三类:假包膜型、假包膜浸润型和假包膜穿透型。实体瘤组假包膜型发生率(18.2%)低于细胞株组(42.3%,P<0.05),而实体瘤组假包膜穿透型发生率(50.0%)高于细胞株组(23.1%,P<0.05),两组间的假包膜浸润型差异无统计学意义(P>0.05)。肿瘤在卵巢内浸润类型随着种植时间而发生变化,种植初期假包膜型比例较高,随着种植时间的延长,假包膜穿透型比例增加。人卵巢上皮癌卵巢内浸润类型中,Ⅰ期假包膜型比例较高(P<0.05),而Ⅱ期包膜穿透型比例较高(P<0.05)。两种病理类型之间其肿瘤生长类型的比例差异无统计学意义(P>0.05)。结论人卵巢上皮癌实体瘤组织片和人卵巢上皮癌细胞株OVCAR-3制备裸鼠原位移植瘤模型有差别;相对于实体瘤组织片模型,细胞株模型更稳定,更适合后续研究。三种肿瘤卵巢内浸润类型在移植瘤种植8周时比例比较均衡,8周是后续实验最佳切入时机。Objective The aim of this study was to study the characteristics of neoplasm invasion type in ovary of two orthotopic models established with human epithelial ovarian cancer solid tumor tissue slices and human ovarian carcinoma cell line OVCAR-3 in nude mice and human epithelial ovarian cancer.Methods Tumor tissues and cell line OVCAR-3 of human epithelial ovarian cancer were grown in subcutaneous tissue and the subcutaneous tumor source was fetched and inoculated in ovarian capsule of nude mice to establish the orthotopic implantation model.The neoplasm invasion type in the two kinds of models were observed.The neoplasm invasion types were also analyzed by pathological examination in 54 cases of International Federation of Gynecology and Obstetrics(FIGO)stageⅠ-Ⅱepithelial ovarian cancer.Results Three neoplasm invasion types were found as follows:type of pseudocapsule,type of pseudocapsule invasion,type of pseudocapsule penetration.Pseudocapsule rate in the solid tumor slices group(18.2%)were lower than those in the cell line group(42.3%)(P<0.05),while the pseudocapsule penetration rate in the solid tumor slices(50.0%)were higher than those in the cell line group(23.1%)(P<0.05).No difference was found of pseudocapsule invasion rate between two groups(P>0.05).Neoplasm invasion type in ovary changed with tumor planting time.High proportion of pseudocapsule type was found at the beginning of tumor planting,and the pseudocapsule penetration rate raised with tumor planting time increased.High proportion of pseudocapsule type was also found in patients with FIGO stageⅠepithelial ovarian cancer,and pseudocapsule penetration rate increased in those with FIGO stageⅡ.No difference in neoplasm invasion type was found between two kinds of pathological types(P>0.05).Conclusions There are differences between the two kinds of orthotopic models established with human epithelial ovarian cancer solid tumor tissue slices and human ovarian carcinoma cell line OVCAR-3.Compared to the solid tumor slices model,the cell line mod

关 键 词：小鼠 裸 人卵巢上皮癌 卵巢原位移植瘤 肿瘤细胞 培养的 肿瘤浸润 

分 类 号：R737.31[医药卫生—肿瘤]