结直肠癌患者循环微小RNA-375和组织Yes相关蛋白1表达的关系及其临床意义  

Expression and clinical significance of circulating microRNA-375 and tissue Yes-associated protein 1 in colorectal cancer patients

作　　者：张斌忠[1] 何春华[1] 王胤达 董来荣[1] Zhang Binzhong;He Chunhua;Wang Yinda;Dong Lairong(Department of Oncological Surgery,the Second Affiliated Hospital of Jiaxing University,Jiaxing 314000,China)

机构地区：[1]嘉兴学院附属第二医院肿瘤外科,314000

出　　处：《中华实验外科杂志》2020年第1期144-147,共4页Chinese Journal of Experimental Surgery

基　　金：嘉兴市科技计划项目(2017BY18017、2018AY32005)。

摘　　要：目的分析结直肠癌患者循环微小RNA(miR-375)和组织Yes相关蛋白1(YAP1)表达的相关性,探讨两者与临床病理特征的关系。方法以嘉兴学院附属第二医院2016年6月至2017年12月收治的87例结直肠癌患者[男45例,女42例,年龄(64.5±11.4)岁]为研究对象,以80例健康志愿者为对照,采用荧光定量聚合酶链反应(PCR)实验测定外周血miR-375表达水平,采用免疫组织化学实验测定结直肠癌病理组织和癌旁健康组织YAP1表达水平,分析两项指标与临床病理特征及预后的关系,进一步根据miR-375和YAP1的表达情况将研究组分为低miR-375且高YAP1组(A组,n=21),中间组(B组,n=32,包含低miR-375且低YAP1,高YAP1且高miR-375两种情况),高miR-375且低YAP1组(C组,n=34),分析两项指标与患者预后的关系。应用SPSS 22.0统计软件分析。结果结直肠癌患者循环miR-375表达水平为0.132±0.085,低于对照组(0.521±0.186),癌组织YAP1表达水平为2.201±0.856,明显高于癌旁健康组织(0.814±0.262),差异有统计学意义(t=51.826、51.175,P<0.05)。miR-375表达和YAP1表达之间呈负相关(r=-0.691,P<0.05),双荧光素酶实验报告证实两者具有相互作用,差异有统计学意义(t=9.564,P<0.05)。miR-375和YAP1表达与Dukes分期明显相关,C期患者miR-375表达低于A和B期,YAP1表达高于A和B期,差异有统计学意义(F=4.347、3.724,P<0.05)。miR-375联合YAP1检查有助于预测结直肠癌复发/转移和死亡(AUC=0.739、0.699,P<0.05),差异有统计学意义,其中低miR-375且高YAP1者中位生存期最短,高miR-375且低YAP1者中位生存期最长,组间差异有统计学意义(χ^2=4.685,P<0.05)。结论miR-375和YAP1存在交互作用,两者共同参与结直肠癌的发生与发展。Objective To explore the relationship between circle microRNA(miRNA,miR)-375 and tissue Yes-associated protein 1(YAP1)expression in patients with colorectal cancer,as well as their relationship with clinicopathological characteristics of colorectal cancer.Methods Eighty-seven patients with colorectal cancer were selected as the study group,and 80 healthy volunteers served as the control group.The circle miR-375 expression in both groups was detected by quantitative polymerase chain reaction(PCR).The YAP1 expression in both cancer tissue and adjacent healthy tissue was detected by immunohistochemistry.The relationship between the two indexes and clinicopathological characteristics and prognosis was analyzed.The patients in the study group were divided into 3 groups according to miR-375 and YAP1 expression:Lower miR-375 and higher YAP1(group A,n=21),both lower miR-375 and YAP1,or both higher miR-375 and YAP1(group B,n=32),and higher miR-375 and lower YAP1(group C,n=34).The relationship between the two indexes andprognosis was analyzed.Results The circle miR-375 expression in case group(0.132±0.085)was significantly lower than that in the control group(0.521±0.186),and the expression level of YAP1 in cancer tissues(2.201±0.856)was significantly higher than that in healthy tissues(0.814±0.262)(t=51.826,51.175,P<0.05).There was a linear negative correlation between miR-375 and YAP1 expression(r=-0.691,P<0.05),and the interaction between them was confirmed in double luciferase assay report(t=9.564,P<0.05).The expression of both miR-375 and YAP1 was related to Dukes stage,and the miR-375 expression in patients with colorectal cancer of stage C was significantly lower than that in stage A and stage B,and the YAP1 expression in stage C was significantly higher than that in stage A and stage B(F=4.347,3.724,P<0.05).The detection of miR-375 combined with YAP1 showed good predictive value for recurrence/metastasis and death of colorectal cancer(AUC=0.739,0.699,P<0.05).The patients in group A got the shortest median survi

关 键 词：结直肠癌 微小RNA-375 Yes相关蛋白1 Hippo信号通路 预后 

分 类 号：R735[医药卫生—肿瘤]