切除修复交叉互补基因1和切除修复交叉互补基因2基因多态性与膀胱癌易感性的关系  

Correlation between gene polymorphisms of excision repair cross-complementing gene 1 and excision repair cross-complementing gene 2 and susceptibility to bladder cancer in Chinese population

作　　者：徐海亮 朱海松 景中民 李军 夏明亮 Xu Hailiang;Zhu Haisong;Jing Zhongmin;Li Jun;Xia Mingliang(Department of Urology,Zhumadian Central Hospital,Zhumadian 463000,China)

机构地区：[1]河南省驻马店市中心医院泌尿外科,463000

出　　处：《中华实验外科杂志》2020年第1期162-165,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨切除修复交叉互补基因(ERCC)1(rs3212986)和ERCC2(rs13181)基因单核苷酸多态性与中国人群膀胱癌易感性的关系。方法选取2015年3月至2019年3月驻马店市中心医院194例膀胱癌患者(实验组)和240例健康人群(对照组)作为研究对象。病例组男143例,女51例;<50岁85例,≥50岁109例;体重指数(BMI)<25 kg/m2154例,BMI≥25 kg/m240例;对照组中男145例,女95例;<50岁121例,≥50岁119例;BMI<25 kg/m2201例,BMI≥25 kg/m239例。采用聚合酶链式反应限制性片段长度多态性(PCR-RFLP)技术检测ERCC1 rs3212986和ERCC2 rs13181位点的基因型,探讨各基因型与膀胱癌发病风险的关系,应用SPSS 22.0统计软件分析。结果两组间ERCC1 rs3212986基因型分布差异有统计学意义(χ^2=6.010,P<0.05),无条件Logistic回归分析显示,ERCC1 rs3212986的CC基因型携带者发生膀胱癌的风险是携带AA基因型携带者的2.05倍[校正比值比(OR)=2.05,95%可信区间(CI):1.10~3.83,P<0.05],差异有统计学意义;ERCC1 rs3212986的CC基因型携带者发生膀胱癌的风险是AA+AC基因型携带者的1.8倍(校正OR=1.80,95%CI:1.01~3.21,P<0.05),差异有统计学意义,ERCC2 rs13181单核苷酸多态性与膀胱癌易感性之间无明显相关(χ^2=0.230,P>0.05)。结论ERCC1 rs3212986基因多态性影响共显性和隐性模型中膀胱癌的发生,ERCC2 rs13181基因多态性与膀胱癌的发生风险无明显相关。Objective To investigate the relationship between single nucleotide polymorphisms of excision repair cross-complementing gene(ERCC)1(rs3212986)and ERCC2(rs13181)gene and bladder cancer susceptibility in Chinese population.Methods A total of 194 patients with bladder cancer(case group)and 240 healthy subjects(control group)were enrolled.In the case group,there were 143 males and 51 females,85 cases under 50 years old and 109 cases over 50 years old,body mass index(BMI)<25154 cases and BMI≥2540 cases,119 cases without smoking history and 75 cases with smoking history,122 cases without drinking history and 72 cases with drinking history,179 cases without family tumor history and 15 cases with family tumor history.In the control group,there were 145 males and 95 females;121 cases under 50 years old and 119 cases over 50 years old,BMI<25201 cases and BMI≥2539 cases,176 cases without smoking history and 64 cases with smoking history,169 cases without drinking history and 71 cases with drinking history,224 cases without family tumor history and 16 cases with family tumor history.The genotypes of ERCC1 rs3212986 and ERCC2 rs13181 were detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).The relationship between each genotype and the risk of bladder cancer was explored.Results There was a statistically significant difference in the distribution of ERCC1 rs3212986 genotype between the two groups(χ^2=6.010,P<0.05).Unconditional Logistic regression analysis showed that the risk of bladder cancer in CC genotype carriers of ERCC1 rs3212986 was 2.05 times higher than that of carriers carrying AA genotype[corrected odds ratio(OR)=2.05,95%confidence interval(CI):1.10-3.83,P<0.05].The risk of bladder cancer in CC genotype carriers of ERCC1 rs3212986 was 1.8 times higher than that of AA+AC genotype carriers(corrected OR=1.80,95%CI:1.01-3.21,P<0.05).In addition,there was no significant correlation between ERCC2 rs13181 single nucleotide polymorphism and bladder cancer susceptibility(χ^2=0.23

关 键 词：膀胱癌 基因多态性 切除修复交叉互补基因 

分 类 号：R737[医药卫生—肿瘤]