小鼠动脉粥样硬化模型的建立  

Establishment of mouse atherosclerotic model

作　　者：戴贻权[1] 颜晓晓 刘晓如[3] 林奕辰 陈宏宇[1] Dai Yiquan;Yan Xiaoxiao;Liu Xiaoru;Lin Yichen;Chen Hongyu(Department of Vascular Surgery,the Affiliated First Hospital of Fujian Medical University,Fuzhou 350005,China;Fujian Children’s Hospital,Fuzhou 350122,China;Basic Medical School of Fujian Medical University,Fuzhou 350122,China)

机构地区：[1]福建医科大学附属第一医院血管外科,福州350005 [2]福建省儿童医院,福州350001 [3]福建医科大学基础医学院,福州350122

出　　处：《中华实验外科杂志》2020年第1期172-175,共4页Chinese Journal of Experimental Surgery

基　　金：福建省自然科学基金(2017J01281、2019J01296)。

摘　　要：目的建立稳定可靠的小鼠动脉粥样硬化(AS)模型。方法采用载脂蛋白E(ApoE)-/-小鼠复合高脂饮食诱导小鼠AS模型。随机分成4组:C57BL/6J普通饲料组(C57+nd),C57BL/6J高脂饲料组(C57+hfd),ApoE-/-普通饲料组(ApoE-/-+nd)和ApoE-/-高脂饲料组(ApoE-/-+hfd),喂养16周后通过一般情况、血脂水平、油红O染色和主动脉病理切片苏木精-伊红(HE)染色等检测,鉴定小鼠AS模型。所有数据均以均值±标准差(Mean±SD)表示,各组间均数比较采用One-way ANOVA分析。结果高脂饮食ApoE-/-小鼠血浆中的胆固醇[(20.09±1.02)mmol/L]和低密度脂蛋白胆固醇[(6.84±0.65)mmol/L]水平显著高于普通饮食的C57BL/6J小鼠[总胆固醇(TC):(2.04±0.07)mmol/L,低密度脂蛋白胆固醇(LDL-C):(0.25±0.01)mmol/L,F=190.543、82.795,P<0.01],差异有统计学意义;油红O染色显示主动脉斑块面积占整体主动脉面积[(22.09±3.49)%],显著高于普通饮食ApoE-/-小鼠组[(1.46±0.96)%,F=118.558,P<0.01],差异有统计学意义;主动脉窦切片显示斑块面积约为普通饮食ApoE-/-小鼠组的2倍,主动脉病理切片HE染色呈现典型的AS斑块。结论ApoE-/-小鼠经高脂饮食诱导可成功建立AS模型,并建立基因鉴定、一般情况分析、血脂水平分析、病理学检测一整套基本的模型鉴定流程,为AS相关性疾病的研究提供较为理想的体内研究动物模型。Objective To establish a stable and reliable mouse atherosclerotic(AS)model,and standardize principal process of AS model identification.Methods The mouse AS model was developed by feeding apolipoprotein E(ApoE)gene knockout mice(ApoE-/-)on high-fat diet(hfd).Sixteen weeks later,the body weight,blood sugar,blood pressure and blood lipid were determined,and aortas were stained with oil red O and hematoxylin to evaluate the AS plaque.Results Cholesterol[(20.09±1.02)mmol/L]and low density lipoprotein cholesterol[(6.84±0.65)mmol/L]of ApoE-/-mice with hfd were significantly increased as compared with that in C57BL/6J normal diet(nd)group[total cholesterol(TC):(2.04±0.07)mmol/L,LDL-C:(0.25±0.01)mmol/L,F=190.543,82.795,P<0.01].Oil Red O staining of entire aorta showed that AS lesion size in ApoE-/-+hfd mice[(22.09±3.49)%]was dramatically increased as compared with that in ApoE-/-+nd mice[(1.46±0.96)%,F=118.558,P<0.01].Similar result was obtained from cross-sections of aortic root analysis.Hematoxylin and eosin(HE)staining of cross-sections of aorta root showed typical As plaques.Conclusion The hfd treatment successfully promoted AS development in ApoE-/-mice.The process of gene identification-general situation analysis-blood lipid analysis-morphologic analysis was established to verify AS model.The establishment of mouse AS model provides a valuable tool for the study of AS-related diseases in vivo.

关 键 词：动脉粥样硬化 基因敲除小鼠 低密度脂蛋白 胆固醇 主动脉 

分 类 号：R54[医药卫生—心血管疾病]