土壤中细菌菌落总数的测定条件优化  

Optimizing the Determination Conditions of Total Bacterial Colonies in Soil

作　　者：谢夏 杨建兰 刘新育[1] Xie Xia;Yang Jianlan;Liu Xinyu(Key Laboratory of Enzyme Engineering of Agricultural Microbiology,Ministry of Agriculture,Henan Agricultural University,Zhengzhou 450002)

机构地区：[1]河南农业大学农业部农业微生物酶工程重点实验室,郑州450002

出　　处：《中国农学通报》2020年第11期92-95,共4页Chinese Agricultural Science Bulletin

基　　金：河南省高等学校重点科研项目“黑曲霉GH10木聚糖酶关键氨基酸与小麦XIP抑制蛋白的相互作用研究”(14A180022);NSFC-河南人才培养联合基金项目“小麦饲料中木聚糖酶抑制蛋白对外加木聚糖酶的作用研究”(U1404324)。

摘　　要：菌落计数法是土壤中可培养微生物总数测定的常用方法,但其重复性差。本文研究了试验过程中培养时间、接种方式、样品颗粒大小、玻璃珠数量及培养基种类等因素对菌落计数结果的影响。结果表明:培养时间到第4天后数量逐渐稳定;涂布法接种方式培养得到的菌落总数较高,是混菌法接种的3.11倍;土壤颗粒大小为50目时测定得到的菌落总数最多;用40颗玻璃珠制备土壤浸提液时可培养得到最大菌落总数;采用SOC培养基进行培养获得菌落总数为1.07×10^7CFU/g,分别是LB培养基、TB培养基和异氧细菌培养基的1.28、2.32、1.63倍。The colony counting is a common method for measuring the total number of culturable microorganisms in soil, but with poor repeatability. We studied the effects of culture time, inoculation method,sample particle size, number of glass beads and type of medium on the colony counting results during the experiment. The results showed that the counting number was gradually stabilized after the fourth day of culture;the total number of colonies obtained by the spread inoculation was higher, which was 3.11 times that of the pour inoculation;the maximum total number of colonies was measured when the soil particle size was 50 mesh. The maximum number of colonies could be obtained by preparing the soil extract with 40 glass beads.The total number of colonies on SOC medium was 1.07×10^7 CFU/g, which was 1.28 times, 2.32 times and 1.63 times that of the LB medium, TB medium and heterotrophic bacteria medium, respectively.

关 键 词：土壤 菌落总数 稀释平板计数 条件优化 

分 类 号：Q93-332[生物学—微生物学]