银杏叶提取物对H2O2致SH-SY5Y损伤细胞糖代谢的影响  

Effects of Ginkgo biloba extract on glucose metabolism in SH-SY5Y injured cells induced by H 2O 2

作　　者：张徽 王淑静[1] 袁天宇 杨骄 代民言 张琦 金成斌 ZHANG Hui;WANG Shu-jing;YUAN Tian-yu;YANG Jiao;DAI Min-yan;ZHANG Qi;JIN Cheng-bin((School of Pharmacy,Harbin University of Commerce,Harbin 150076,China)

机构地区：[1]哈尔滨商业大学药学院,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2020年第3期259-265,共7页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：哈尔滨商业大学大学生创新创业训练计划项目(201910240001);哈尔滨商业大学研究专项基金资助(18XN049)。

摘　　要：为探究银杏叶提取物对H2O2诱导SH-SY5Y损伤细胞的保护作用及对糖代谢的影响,采用MTT法筛选H2O2的造模浓度和银杏叶提取物的给药浓度;生长曲线法检测银杏叶提取物对SH-SY5Y的增殖影响;HE染色法检测细胞形态;流式细胞仪PI单染检测细胞周期;流式细胞仪Annexin V单染检测细胞凋亡率;分光光度法检测细胞中己糖激酶(HK)、丙酮酸激酶(PK)、琥珀酸脱氢酶(SDH)、苹果酸脱氢酶(MDH)活力和乳酸(LD)、ATP的含量.实验结果表明,H2O2造模浓度为1. 0 mmol/L,银杏叶提取物给药浓度为100μmol/L,银杏叶提取物可通过提高HK、PK、SDH、MDH活力和增加LD、ATP的含量促进SH-SY5Y损伤细胞增殖、抑制凋亡,从而保护神经.To investigate the protective effect of Ginkgo biloba extract on SH-SY5Ycell damage induced by H 2O 2 and its effect on glucose metabolism.MTT method was used to screen the modeling concentration of H 2O 2 and the dosage concentration of Ginkgo biloba extract;.the growth curve was used to detect the protective effect of Ginkgo biloba extract on SH-SY5Y.HE staining was used to detect cell morphology.Flow cytometry PI single staining to detect cell cycle;flow cytometry Annexin V single staining to detect apoptosis rate;the spectrophotometry was used to detect the enzymatic activity of hexokinase(HK),pyruvate kinase(PK),succinate dehydrogenase(SDH),malate dehydrogenase(MDH)and the content of lactic acid(LD)and ATP.The experimental results showed that the final H 2O 2 modeling concentration was 1.0 mmol/L,the final Ginkgo biloba extract concentration was 100μmol/L.Ginkgo biloba extrac can be promoted the proliferation of SH-SY5Y damaged cells and inhibit apoptosis by increasing the enzymatic activity of HK,PK,SDH,MDH and increasing the content of LD and ATP,thereby protecting the nerves.

关 键 词：银杏叶提取物 SH-SY5Y H2O2 氧化损伤 神经保护 糖代谢 ATP 

分 类 号：R730[医药卫生—肿瘤]