肿瘤相关成纤维细胞对卵巢癌细胞增殖作用影响的研究  

Cancer-Associated Fibroblasts Promote Proliferation of Ovarian Cancer Cells

作　　者：赵宗霞[1,3] 苏玉强 曾娟[1] 双婷[4] 陈必良 Zhao Zongxia;Su Yuqiang;Zeng Juan;Shuang Ting;Chen Biliang(Department of Gynecology and Obstetrics,Second Hospital Affiliated to Xi’an Medical University,Xi’an 710038,Shaanxi,China;Department of Anesthesiology,Second Hospital Affiliated to Xi’an Medical University,Xi’an 710038,Shaanxi,China;Department of Gynecology and Obstetrics,Xijing Hospital,Air Force Medical University,Xi’an 710032,Shaanxi,China;Department of Gynecology and Obstetrics,First Affiliated Hospital of Xi’an Jiao Tong University,Xi’an 710000,Shaanxi,China)

机构地区：[1]西安医学院第二附属医院妇产科,西安710038 [2]西安医学院第二附属医院妇产科麻醉科,西安710038 [3]第四军医大学西京医院妇产科,西安710032 [4]西安交通大学第一附属医院妇产科,西安710000

出　　处：《肿瘤预防与治疗》2020年第5期383-388,共6页Journal of Cancer Control And Treatment

基　　金：国家自然科学基金项目(编号:81702555)。

摘　　要：目的:探讨肿瘤相关成纤维细胞(cancer-associated fibroblast,CAF)分泌的肝细胞生长因子(hepatocyte growth factor,HGF)对卵巢癌SKOV3细胞增殖作用的影响。方法:选取在本院手术的卵巢癌患者及其他妇科疾病同时切除正常卵巢手术患者,收集卵巢癌组织、癌旁组织和正常组织,免疫组化观察卵巢癌组织、癌旁组织和正常组织中CAF标志蛋白α平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)和I型胶原蛋白表达情况;用卵巢癌相关成纤维细胞(human endometrial carcinoma-1A cell,HEC-1A)培养48小时后的培养上清,培养卵巢癌细胞SKOV3行细胞计数试剂盒-8(Cell Counting Kit-8,CCK-8)增殖实验、克隆形成实验和倒置显微镜观察检测使用HEC-1A培养上清后SKOV3细胞增殖能力变化;酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)检测HEC-1A与SKOV3细胞共培养48小时后的培养上清中HGF的表达。结果:免疫组化结果显示,卵巢癌组织中α-SMA和I型胶原蛋白的表达要明显高于癌旁组织和正常组织;CCK-8增殖实验和克隆形成实验结果显示HEC-1A培养上清处理后的SKOV3细胞的增殖能力显著增强;细胞形态学和免疫荧光结果提示HEC-1A促进SKOV3上皮样细胞增多;ELISA结果显示HEC-1A与SKOV3细胞共培养48小时后的培养上清中HGF的表达明显升高。结论:肿瘤相关成纤维细胞可能通过分泌HGF诱导因子促进卵巢癌细胞的增殖作用。Objective:To investigate the effect of hepatocyte growth factor(HGF)secreted by cancer-associated fibroblast(CAF)on the proliferation of ovarian cancer SKOV3 cells.Methods:Patients with ovarian cancer or other gynecological diseases undergoing oophorectomy in our hospital were selected.Ovarian cancer tissue,paracancer tissue and normal ovarian tissue were collected.Immunohistochemical staining was used to analyze the expression ofα-SMA and Collagen I,CAF-specific markers,in ovarian cancer tissue,paracancer tis-sue and normal ovarian tissue.Ovarian cancer SKOV3 cells cultured by the supernatant of HEC-1A,a CAF,were used for Cell Counting Kit-8(CCK-8)assay and colony formation assay.Inverted microscope was used to detect the proliferation of HEC-1A supernatant-cultured SKOV3 cells.Enzyme linked immunosorbent assay(ELISA)was used to detect the expression of HGF in the supernatant of HEC-1A co-cultured with SKOV3 for 48 hours.Results:Results of immunohistochemistry showed that the expression ofα-SMA and Collagen I in ovarian cancer tissue was significantly higher than that in adjacent tis-sue and normal ovarian tissue.Results of CCK-8 assay and clone formation assay showed that the proliferation of HEC-1A su-pernatant-cultured SKOV3 cells significantly enhanced.Results of cytomorphology and immunofluorescence suggested that HEC-1A promotes proliferation of epithelial-like SKOV3.ELISA results showed that HGF-1A expression significantly in-creased in the supernatant of HEC-1A co-cultured with SKOV3 for 48 hours.Conclusion:CAF may promote the proliferation of ovarian cancer cells by secreting HGF-inducing factors.

关 键 词：肿瘤相关成纤维细胞 卵巢癌 肝细胞生长因子 增殖 

分 类 号：R737.31[医药卫生—肿瘤] R730.2[医药卫生—临床医学]