川参通注射液中总酚酸、总黄酮及单糖大类成分的含量测定  

Determination of Total Phenolic Acids,Flavonoids and Monosaccharide in Chuanshentong Injection

作　　者：何峰 余明丽 姚光哲 张昀 HE Feng;YU Mingli;YAO Guangzhe;ZHANG Yun(School of Pharmacy,Guizhou Medical University,Guiyang 550025,Guizhou,China;Guizhou Ruihe Pharmaceutical Co.,Ltd,Guiyang 550002,Guizhou,China)

机构地区：[1]贵州医科大学药学院,贵州贵阳550025 [2]贵州瑞和制药有限公司,贵州贵阳550002

出　　处：《贵州医科大学学报》2020年第6期668-671,688,共5页Journal of Guizhou Medical University

基　　金：黔南州工业计划项目[黔南科合工字(2016)9]。

摘　　要：目的:建立川参通注射液中总酚酸、总黄酮及单糖三大类成分含量的测定方法。方法:取10批次川参通注射液成品,采用紫外分光光度法对其中总酚酸、总黄酮和单糖大类成分进行线性、精密度、稳定性、重现性及加样回收率等方法学考察,并测定各类成分的含量。结果:总酚酸、总黄酮和单糖大类成分的线性r值均大于0. 999,精密度、稳定性及重现性的相对标准偏差(RSD)<1. 0%,加样回收率介于98%~102%,10批川参通注射液中总酚酸、总黄酮及单糖的平均含量分别为2. 05、18. 21及53. 58 g/L。结论:所建立的川参通注射液中总酚酸、总黄酮及单糖大类成分的含量测定方法简便、准确可靠。Objective:To establish a measuring method to determine the volume of total phenolic acids,flavonoids and monosaccharide in chuanshentong injection.Methods:By means of UV spectrophotometry,the linearity,precision,stability,reproducibility and the sample recovery rate of the concentrations of total phenolic acids,flavonoids and monosaccharide were determined in 10 batches of chuanshentong injection.Results:The correlation coefficient r of total phenolic acids,flavonoids and monosaccharide were all greater than 0.999.The RSD of precision,stability and reproducibility was all less than 1.0%.The recovery rates were between 98%and 102%.The average concentrations of total phenolic acids,flavonoids,and monosaccharides in 10 batches of chuanshentong injection were 2.05,18.21 and 53.58 g/L respectively.Conclusion:The measuring method is simple,accurate and reliable for the determination of the concentration of total phenolic acids,flavonoids and monosaccharide in chuanshentong injection.

关 键 词：中草药 川参通注射液 大类成分 总酚酸 总黄酮 单糖 紫外分光光度法 

分 类 号：R914.1[医药卫生—药物化学]