葡糖醋杆菌J2-1静态发酵生产细菌纤维素的培养基优化  

Medium optimization of bacterial cellulose production by Gluconacetobacter J2-1 with static fermentation

作　　者：赵航 陈沙 张璇 陈桂光[1] 梁智群[1] 龙晓鹏 ZHAO Hang;CHEN Sha;ZHANG Xuan;CHEN Guiguang;LIANG Zhiqun;LONG Xiaopeng(Guangxi Research Center of Microorganism and Enzyme Engineering Technologyy College of Life Science and Technology,Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学生命科学与技术学院广西微生物与酶工程技术研究中心,广西南宁530004

出　　处：《中国酿造》2020年第5期152-156,共5页China Brewing

基　　金：国家自然科学基金资助项目(31560448)。

摘　　要：为提高葡糖醋杆菌(Gluconacetobacter)J2-1发酵生产细菌纤维素的产量,采用静态发酵方式,利用单因素试验对发酵培养基的碳源、氮源、乙醇、有机酸及无机盐进行优化,并在此基础上选取葡萄糖、Mg SO4·7H2O和酵母粉添加量进行正交试验优化。结果表明,发酵培养基最优组分为:葡萄糖80 g/L、酵母粉18 g/L、乙醇2%(V/V)、Na2HPO4·12H2O 3 g/L、乳酸2 g/L、Mg SO4·7H2O 0.4 g/L。在此优化发酵培养基条件下,葡糖醋杆菌J2-1静态发酵生产细菌纤维素产量达到9.34 g/L,是优化前的1.89倍。In order to improve the bacterial cellulose production by Gluconacetobacter J2-1,the carbon source,nitrogen source,ethanol,organic acid and inorganic salt of fermentation medium were optimized by single factor experiments under static fermentation.On the basis of single factor experiments results,the glucose,MgSO4·7H2O and yeast extract powder addition were optimized by orthogonal tests.The results showed that the optimal fermentation medium components were glucose 80 g/L,yeast extract powder 18 g/L,ethanol 2%(V/V),Na2HPO4·12H2O 3 g/L,lactic acid 2 g/L,and Mg SO4·7H2O 0.4 g/L.Under the optimal fermentation medium conditions,the production of bacterial cellulose by Gluconacetobacter J2-1 with static fermentation reached 9.34 g/L,which was 1.89 times that before optimization.

关 键 词：细菌纤维素 葡糖醋杆菌 培养基优化 

分 类 号：Q8[生物学]