Wnt诱导分泌蛋白-2对胃癌细胞增殖、凋亡和迁移的影响及其机制  

Effect of Wnt-induced signaling protein-2 on proliferation,apoptosis and migration of gastric cancer cells

作　　者：崔婷婷[1] 闫文锋[2] 石书伟[3] 常文娟[4] Cui Tingting;Yan Wenfeng;Shi Shuwei;Chang Wenjuan(Department of Geriatrics,Jiaozuo People’s Hospital,Jiaozuo 454002,China;Department of Gastrointestinal Surgery,Henan Provincial People’s Hospital,Zhengzhou 450000,China;Department of General Surgery,Jiaozuo People’s Hospital,Jiaozuo 454002,China;Department of Gastroenterology,Jiaozuo People’s Hospital,Jiaozuo 454002,China)

机构地区：[1]焦作市人民医院老年医学科,454002 [2]河南省人民医院胃肠外科,郑州450003 [3]焦作市人民医院普外三区,454002 [4]焦作市人民医院消化内科,454002

出　　处：《中华实验外科杂志》2020年第3期416-419,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察Wnt诱导分泌蛋白-2(WISP-2)对胃癌细胞增殖、凋亡和迁移的影响并探讨其分子机制。方法选取2015年6月到2018年6月收集的112例胃癌组织和对应的癌旁组织作为研究对象。采用免疫组织化学观察胃癌组织和癌旁组织中WISP-2蛋白的表达。采用携带WISP-2基因和空载体的慢病毒感染胃癌细胞株MKN-28,建立WISP-2过表达细胞株(WISP-2组)和对照细胞株(对照组)。采用细胞计数试剂盒(CCK-8)分析两组细胞的增殖能力;采用异种肿瘤移植模型分析两组细胞在体内的生长;采用流式细胞术分析两组细胞的凋亡水平;采用Transwell分析两组细胞的迁移能力。采用蛋白质印迹法(Western blot)分析两组细胞WISP-2蛋白、凋亡相关蛋白与迁移相关蛋白的表达。采用t检验和方差分析。结果胃癌组织中WISP-2蛋白的表达水平(89.43±7.51)较癌旁组织中WISP-2蛋白表达水平(193.42±12.09)显著下调,差异有统计学意义(t=6.019,P<0.05)。WISP-2组细胞增殖(1.21±0.14)明显低于对照组(1.79±0.21),差异有统计学意义(t=2.916,P<0.05)。WISP-2组细胞在裸鼠中的肿瘤体积[(1029.32±101.58)mm3]明显小于对照组[(1930.60±198.44)mm3],差异有统计学意义(t=3.001,P<0.05)。WISP-2组细胞凋亡水平(21.45±4.91)较对照组(3.41±0.56)显著增加,差异有统计学意义(t=2.819,P<0.05)。WISP-2组细胞迁移数量[(231.23±19.32)个]明显低于对照组细胞迁移数量[(98.21±10.32)个],差异有统计学意义(t=4.013,P<0.05)。WISP-2组细胞半胱氨酰天冬氨酸特异性蛋白酶(Caspase)-3和B细胞淋巴瘤/白血病-2相关X蛋白(bax)蛋白表达水平(1.94±0.34、1.53±0.19)较对照组Caspase-3和bax表达水平(0.98±0.24、0.55±0.10)显著增加,差异有统计学意义(t=2.193、3.018,P<0.05)。WISP-2组细胞黏着斑激酶(FAK)蛋白表达水平(0.58±0.10)较对照组细胞FAK蛋白表达水平(1.27±0.17)显著下降,差异有统计学意义(t=2.990,P<0.05)。结论WISP-2蛋白在胃癌组Objective To investigate the effect of Wnt-induced signaling protein-2(WISP-2)on the proliferation,apoptosis and migration of gastric cancer cells and its molecular mechanism.Methods 112 cases of gastric cancer tissues and corresponding adjacent tissues collected from June 2015 to June 2018 were selected as the study subjects.The expression of WISP-2 protein in gastric cancer and adjacent tissues was analyzed by immunohistochemistry.The gastric cancer cell line MKN-28 was infected by lentivirus carrying WISP-2 gene and empty vector.The overexpression cell line of WISP-2(WISP-2 group)and the control cell line(control group)were established.The proliferation ability of the two groups was analyzed by cell counting kit-8(CCK-8).The tumor growth of WISP-2 group and control group in vivo was analyzed by xenogeneic tumor transplantation model.The apoptosis level in WISP-2 group and control group was analyzed by flow cytometry.The migration ability in WISP-2 group and control group was analyzed by Transwell assay.The expression of WISP-2 protein,apoptosis associated proteins and migration associated protein in the two groups was analyzed by Western blotting.Results Compared with the paracancerous tissues(193.42±12.09),the expression level of WISP-2 protein in the gastric cancer tissues(89.43±7.51)was significantly down-regulated(t=6.019,P<0.05).The cell proliferation of WISP-2 group(1.21±0.14)was significantly lower than that in the control group(1.79±0.21)(t=2.916,P<0.05).The tumor volume in WISP-2 group[(1029.32±101.58)mm3]was significantly smaller than that in control group[(1930.60±198.44)mm3,t=3.001,P<0.05].As compared with the control group(3.41±0.56),the apoptosis level of WISP-2 group(21.45±4.91)significantly increased(t=2.819,P<0.05).The number of migrating cells in WISP-2 group[(231.23±19.32)cells]was significantly reduced as compared with that in the control group[(98.21±10.32)cells,t=4.013,P<0.05].As compared with the control group(0.98±0.24,0.55±0.10),the expression levels of Caspase-3 and bax in

关 键 词：Wnt-1诱导分泌蛋白2 胃癌 增殖 凋亡 迁移 

分 类 号：R735.2[医药卫生—肿瘤]