混合性谱系激酶-1在胃癌组织的表达及其临床意义  

Mixed lineage kinase 1 is a novel prognostic factor for gastric cancer

作　　者：罗俊[1] 王海峰[2] 马阿火[1] Luo Jun;Wang Haifeng;Ma Ahuo(Department of Gastroenterology,Shaoxing People’s Hospital,Shaoxing 312000,China;Department of Gastrointestinal Surgery,Shaoxing People’s Hospital,Shaoxing 312000,China)

机构地区：[1]绍兴市人民医院消化内科,312000 [2]绍兴市人民医院胃肠外科,312000

出　　处：《中华实验外科杂志》2020年第3期423-425,共3页Chinese Journal of Experimental Surgery

基　　金：绍兴市卫生计生科技计划青年科技项目(2016QN001);绍兴市科技计划(医卫类)项目(2018C30097)。

摘　　要：目的观察混合性谱系激酶-1(MLK1)在胃癌组织的表达特点,探讨MLK1对胃癌患者生存的影响,并分析其与患者基本临床病理特征的关系。方法在Oncomine数据库中的376个分析中检索MLK1的分布,在20个涉及胃癌的分析中研究MLK1在胃癌组织和正常组织的差异性表达;在Kaplan-Meier Plotter在线数据库中,分析2018年1月至2019年2月875例胃癌患者组织中的MLK1的mRNA水平对胃癌患者多种生存的影响;收集绍兴市人民医院75例胃癌患者癌组织新鲜标本,并行实时定量聚合酶链反应(Real-time PCR)检测MLK1的mRNA表达水平,进行患者基本临床病理特征的相关性分析。结果在Oncomine数据库中,胃癌组织的MLK1的表达水平是胃正常组织中的3.104倍(t=4.709,P<0.05);胃癌组织中MLK1表达较高的胃癌患者的总生存(OS)、疾病进展后生存(PPS)、首次进展后生存(FP)显著低于MLK1低表达胃癌人群[风险比(HR)=1.89(1.58~2.27)、1.8(1.43~2.28)、1.85(1.49~2.31),P<0.05];MLK1表达较高患者中,癌组织的分化程度较低(χ2=4.208,P<0.05),较易出现淋巴结转移(χ2=6.299,P<0.05),局部浸润也较深(χ2=9.255,P<0.01),差异均有统计学意义。结论胃癌患者癌组织中MLK1表达水平高于正常组织,癌组织MLK1高表达患者的生存较差,MLK1与胃癌分化程度、局部浸润深度和局部淋巴结转移显著相关。Objective To evaluate the expression levels of mixed lineage kinase 1(MLK1)in gastric cancer and analyze the correlation of MLK1 and clinicopathological characteristics of gastric cancer,and its effects on overall survival(OS),post-progression survival(PPS)and first progression(FP)in gastric cancer.Methods In Oncomine database(20 in 376 analyses),the expression levels of MLK1 in gastric cancer samples and normal tissues were evaluated.Real-time quantitative polymerase chain reaction(Real-time PCR)was carried out to detect the RNA levels of MLK1 in gastric cancer tissues and the correlation of MLK1 and the clinicopathological characteristics of gastric cancer was evaluated.Online KM database was used to test the effects of MLK1 on gastric cancer survival in 875 patients.Results The MLK1 levels were significantly higher in cancer tissues than in normal tissues in gastric caner(fold change 3.104,t=4.709,P<0.05).MLK1 levels were correlated to differentiation negatively,but positively to tumor infiltration and lymph node metastasis(χ2=4.208,9.255 and 6.299,P<0.05,0.01,0.05).OS,PPS and FP rates in gastric cancer patients with higher levels of MLK1 were significantly lower than those in the patients with lower levels of MLK1 expression[hazard ratio(HR)=1.89(1.58-2.27),1.8(1.43-2.28)and 1.85(1.49-2.31),P<0.05].Conclusion MLK1 was up-regulated in gastric cancer samples,and correlated to differentiation negatively,tumor infiltration and lymph node stage positively.Up-regulated expression of MLK1 is related to shorter survival in gastric cancer.

关 键 词：胃癌 混合性谱系激酶-1 生存 

分 类 号：R735.2[医药卫生—肿瘤]