蜡样芽孢杆菌0-9对小麦根腐病菌的抑菌作用研究  

Study on the Bacteriostatic Effect of Bacillus cereus 0-9 on Root Rot of Wheat

作　　者：黄秋斌 韩兴旺 刘凤英[1] HUANG Qiu-bin;HAN Xing-wang;LIU Feng-ying(School of Life Science,Henan University,Kaifeng,Henan 475004)

机构地区：[1]河南大学生命科学学院,河南开封475004

出　　处：《安徽农业科学》2020年第12期135-138,共4页Journal of Anhui Agricultural Sciences

基　　金：河南省高等学校重点科研项目(16A180021)。

摘　　要：采用平板对峙法,研究0-9菌株对引起小麦根腐病的致病菌尖孢镰刀菌的抑菌作用,抑菌率为50%,该菌株发酵代谢产物对小麦根腐病菌的抑菌作用达83%;0-9菌株代谢产物处理尖孢镰刀菌的分生孢子后,其萌发率降低61.2%;发酵代谢物质在高温和长时间紫外线照射下仍具有约80%的抑菌活性,且代谢产物的有效抑菌活性可以长达14 d。同时采用平板对峙法研究0-9菌株对5株植物病原真菌的抑菌作用,结果表明0-9菌株也具有广泛的抑菌效果。The bacteriostatic effect of strain 0-9 on Fusarium oxysporum,which caused wheat root rot,was studied by plate confrontation method.The bacteriostatic rate of Fusarium oxysporum was 50%.The bacteriostatic effect of fermentation metabolites on Fusarium oxysporum was 83%.After the conidia of Fusarium oxysporum was treated with metabolites of strain 0-9,the germination rate decreased by 61.2%,and the fermentation metabolites still had about 80%bacteriostatic activity under high temperature and long time ultraviolet irradiation,and the effective bacteriostatic activity of metabolites could be as long as 14 days.At the same time,the bacteriostatic effect of strain 0-9 on 5 strains of plant pathogenic fungi in laboratory was studied by plate confrontation method.The results showed that strain 0-9 also had a wide range of bacteriostatic effects.

关 键 词：蜡样芽孢杆菌0-9 代谢产物 小麦尖孢镰刀菌 抑菌活性 

分 类 号：S435.121.47[农业科学—农业昆虫与害虫防治]