LncRNA-ROR抑制结直肠癌细胞转移的作用研究  

Study on the inhibition of LncRNA-ROR cells on colorectal cancer metastasis

作　　者：胡世丰[1] 雷春[2] 施炜[1] 查诚[1] HU Shi-feng;LEI Chun;SHI Wei;ZHA Cheng(Department of Medicine,Tongling Vocational and Technical College,Tongling Anhui 244061;Department of General Surgery,Tongling People′s Hospital,Tongling Anhui 244000,China)

机构地区：[1]铜陵职业技术学院医学系,安徽铜陵244061 [2]安徽省铜陵市人民医院普外科,244000

出　　处：《蚌埠医学院学报》2020年第5期569-571,577,共4页Journal of Bengbu Medical College

基　　金：安徽省高校自然科学研究重点项目(KJ2017A635);安徽省质量工程高水平教学团队项目(2018jxtd122)。

摘　　要：目的:了解LncRNA-ROR在结直肠癌细胞转移中的作用。方法:在前期工作中,已经发现LncRNA-ROR在结直肠癌组织中低表达,并抑制肠癌细胞的增殖。进一步,我们采用Transwell功能研究LncRNA-ROR的过表达对结直肠癌细胞SW620迁移的影响;并采用划痕实验探索LncRNA-ROR过表达后结直肠癌细胞SW620伤痕愈合的变化。结果:SW620细胞在ROR过表达后,穿过人工膜的细胞数减少(P<0.01);在迁移和侵袭实验中,与对照组比较,SW620 LncRNA-ROR穿过Transwell膜的细胞数减少(P<0.01),侵袭能力减弱(P<0.01);划痕实验中,划痕48 h后LncRNA-ROR的细胞间间距大于对照组(P<0.01)。结论:LncRNA-ROR抑制结直肠癌细胞的转移。Objective:To investigate the role of LncRNA-ROR cells in metastasis of colorectal cancer.Methods:The low expression of LncRNA-ROR in colorectal cancer was identified,which could inhibit the proliferation of colorectal cancer cells.The effects of the overexpression of LncRNA-ROR on colorectal cancer SW620 cells metastasis were explored using Transwell assay.The role of the overexpression of LncRNA-ROR on the healing of SW620 cells′scratch was investigated using the wound healing assay.Results:After LncRNA-ROR was overexpressed in SW620 cells,the number of cells passing through the artificial membrane decreased(P<0.01).In the migration and invasion experiments,compared with the control group,the number of cells with SW620 LncRNA-ROR passing the Transwell membrane decreased(P<0.01),and the invasion ability decreased(P<0.01).In the wound heding assay,the intercellular spacing of LncRNA-ROR after 48 h of scratching was greater than that in control group(P<0.01).Conclusions:LncRNA-ROR can inhibit the metastasis of colorectal cancer cells.

关 键 词：结直肠肿瘤 LncRNA-ROR SW620细胞 转移 

分 类 号：R735.3[医药卫生—肿瘤]